Evaluation of exposure reducing measures on parameters of genetic risk in a population occupationally exposed to coal fly ash
| Autor: | J.C.S. Kleinjans, Geja J. Hageman, R.H. Stierum, I.J. Welle, J.G.M. Schreurs, H.J. Albering |
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| Rok vydání: | 1993 |
| Předmět: |
Adult
Male Risk Hypoxanthine Phosphoribosyltransferase Population Industrial Waste Mutagen Urine Gene mutation Biology Toxicology medicine.disease_cause Coal Ash Ames test Excretion Animal science Occupational Exposure Genetics medicine Humans Lymphocytes education Micronuclei Chromosome-Defective education.field_of_study Smoking fungi Carbon Coal Fly ash Micronucleus test Regression Analysis Particulate Matter Micronucleus Sister Chromatid Exchange Mutagens |
| Zdroj: | Mutation Research/Genetic Toxicology. 319:245-255 |
| ISSN: | 0165-1218 |
| DOI: | 10.1016/0165-1218(93)90012-3 |
| Popis: | In a previous study we found increased SCE frequencies in peripheral blood lymphocytes (PBLs) of workers occupationally exposed in a coal fly ash processing industry, as compared to a non-exposed control population. Shortly after this study, measures were taken in this plant to reduce fly ash levels. The objective of the present study, conducted 2 years later in the same plants, was to evaluate the effect of these measures with respect to genotoxic risk. A group of 18 male workers of the coal fly ash processing industry agreed to participate in the study. The control population consisted of 18 male workers from a flour processing industry, who were matched for age and smoking behavior. In contrast to our previous study, no increased SCE frequencies were found in PBLs of workers potentially exposed to coal fly ash when compared to the control group (mean SCEs: 6.4 +/- 1.2 and 7.0 +/- 0.9, respectively). In addition, no differences were observed between the exposed and control groups for frequencies of gene mutations at the hypoxanthine guanine phosphoribosyltransferase (hprt) locus in PBLs, for micronucleus frequencies using the cytokinesis block method, or for urinary mutagen excretion measured with Salmonella typhimurium tester strains TA98 and TA97 with and without metabolic activation. In smokers, however, SCE frequencies in PBLs were significantly increased in comparison to non-smokers (7.1 +/- 1.1 vs. 6.1 +/- 0.5; P0.005), as was 24-h urinary mutagen excretion measured with strain TA98 with S9 mix (2373 +/- 1870 vs. 156 +/- 211; P0.001) and with TA98 with S9 mix and beta-glucuronidase/arylsulfatase (2361 +/- 1958 vs. 538 +/- 396; P0.005). In addition, hprt variant frequencies in PBLs were higher in smokers than in non-smokers (15.0 +/- 23.5 x 10(-6)6 vs. 2.6 +/- 2.8 x 10(-6); P0.05). No differences were observed for micronucleus induction between smokers and non-smokers. It is concluded that the protective measures taken in the coal fly ash processing plant appear to have been sufficient, since an effect of exposure to coal fly ash on parameters of genetic risk was not found any longer. |
| Databáze: | OpenAIRE |
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