Long-lasting effects of antibiotics on bacterial communities of adult flies
| Autor: | Christophe Mougel, Maxime R. Hervé, Lionel Lebreton, Anne-Marie Cortesero, Yannick Outreman, Denis Poinsot, Valérie Lopez, Morgane Ourry |
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| Přispěvatelé: | Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique, INRARégion BretagneMP-P10026 EB06, Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
antibiotic resistance
medicine.drug_class Tetracycline media_common.quotation_subject Antibiotics Population Zoology Insect Biology Applied Microbiology and Biotechnology Microbiology Delia radicum 03 medical and health sciences Antibiotic resistance RNA Ribosomal 16S medicine Journal Article Animals Microbiome education cabbage root fly 030304 developmental biology media_common tetracycline generations 0303 health sciences education.field_of_study Bacteria Ecology 030306 microbiology Host (biology) Microbiota Research Support Non-U.S. Gov't fungi biology.organism_classification bacterial communities Anti-Bacterial Agents [SDE]Environmental Sciences medicine.drug |
| Zdroj: | Ourry, M, Lopez, V, Hervé, M, Lebreton, L, Mougel, C, Outreman, Y, Poinsot, D & Cortesero, A M 2020, ' Long-lasting effects of antibiotics on bacterial communities of adult flies ', F E M S Microbiology Ecology, vol. 96, no. 4 . https://doi.org/10.1093/femsec/fiaa028 FEMS Microbiology Ecology FEMS Microbiology Ecology, Wiley-Blackwell, 2020, 96 (4), pp.fiaa028. ⟨10.1093/femsec/fiaa028⟩ FEMS Microbiology Ecology, 2020, 96 (4), pp.fiaa028. ⟨10.1093/femsec/fiaa028⟩ |
| ISSN: | 0168-6496 1574-6941 |
| Popis: | Insect symbionts benefit their host and their study requires large spectrum antibiotic use like tetracycline to weaken or suppress symbiotic communities. While antibiotics have a negative impact on insect fitness, little is known about antibiotic effects on insect microbial communities and how long they last. We characterized the bacterial communities of adult cabbage root fly Delia radicum in a Wolbachia-free population and evaluated the effect of tetracycline treatment on these communities over several generations. Three D. radicum generations were used: the first- and second-generation flies either ingested tetracycline or not, while the third-generation flies were untreated but differed with their parents and/or grandparents that had or had not been treated. Fly bacterial communities were sequenced using a 16S rRNA gene. Tetracycline decreased fly bacterial diversity and induced modifications in both bacterial abundance and relative frequencies, still visible on untreated offspring whose parents and/or grandparents had been treated, therefore demonstrating long-lasting transgenerational effects on animal microbiomes after antibiotic treatment. Flies with an antibiotic history shared bacterial genera, potentially tetracycline resistant and heritable. Next, the transmission should be investigated by comparing several insect development stages and plant compartments to assess vertical and horizontal transmissions of D. radicum bacterial communities. |
| Databáze: | OpenAIRE |
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