Narrow-band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells
| Autor: | Wu‑Qing Wang, Fu‑Guo Zuo, Qin Xiao, Xiao‑Qing Xiao, Jing Wang, Jin‑Feng Wu |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
F-actin rearrangement RHOA Epidermis (botany) biology Melanoma narrow-band ultraviolet B radiation RAC1 Dendrite Articles Melanocyte medicine.disease B16 melanoma cells Cell biology Melanin medicine.anatomical_structure Oncology medicine Ultraviolet light biology.protein dendricity Rac1 |
| Zdroj: | Molecular and Clinical Oncology |
| ISSN: | 2049-9469 2049-9450 |
| Popis: | Melanocytes are found scattered throughout the basal layer of the epidermis. Following hormone or ultraviolet (UV) light stimulation, the melanin pigments contained in melanocytes are transferred through the dendrites to the surrounding keratinocytes to protect against UV light damage or carcinogenesis. This has been considered as a morphological indicator of melanocytes and melanoma cells. Small GTPases of the Rho family have been implicated in the regulation of actin reorganization underlying dendrite formation in melanocytes and melanoma cells. It has been proven that ultraviolet light plays a pivotal role in melanocyte dendrite formation; however, the molecular mechanism underlying this process has not been fully elucidated. The effect of small GTPases, such as Rac1 and RhoA, on the morphology of B16 melanoma cells treated with narrow-band UVB radiation was investigated. The morphological changes were observed under a phase contrast microscope and the F-actin microfilament of the cytoskeleton was observed under a laser scanning confocal microscope. The pull-down assay was performed to detect the activity of the small GTPases Rac1 and RhoA. The morphological changes were evident, with globular cell bodies and increased numbers of tree branch-like dendrites. The cytoskeletal F-actin appeared disassembled following narrow-band UVB irradiation of B16 melanoma cells. Treatment of B16 melanoma cells with narrow-band UVB radiation resulted in the activation of Rac1 in a time-dependent manner. In conclusion, the present study may provide a novel method through which narrow-band UVB radiation may be used to promote dendrite formation by activating the Rac1 signaling pathway, resulting in F-actin rearrangement in B16 melanoma cells. |
| Databáze: | OpenAIRE |
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