The three-dimensional structure of human interphase chromosomes is related to the transcriptome map
| Autor: | Hinco J. Gierman, Sandra Goetze, Vladan Ondrej, Osdilly Giromus, Julio Mateos-Langerak, Rogier Versteeg, Mireille H. G. Indemans, Roel van Driel, Wim de Leeuw, Jan Koster |
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| Přispěvatelé: | Oncogenomics, Cancer Center Amsterdam, Synthetic Systems Biology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Transcription
Genetic Biology Genome Cell Line Cell Line Tumor Image Processing Computer-Assisted Chromosomes Human Humans Molecular Biology Gene Interphase In Situ Hybridization Fluorescence Oligonucleotide Array Sequence Analysis Regulation of gene expression Genetics Models Genetic Genome Human Physical Chromosome Mapping Chromosome Cell Biology Articles Chromatin Evolutionary biology Chromosome Territory Human genome HeLa Cells |
| Zdroj: | Molecular and cellular biology, 27(12), 4475-4487. American Society for Microbiology Molecular and Cellular Biology, 27(12), 4475-4487. American Society for Microbiology |
| ISSN: | 0270-7306 |
| Popis: | The three-dimensional (3D) organization of the chromosomal fiber in the human interphase nucleus is an important but poorly understood aspect of gene regulation. Here we quantitatively analyze and compare the 3D structures of two types of genomic domains as defined by the human transcriptome map. While ridges are gene dense and show high expression levels, antiridges, on the other hand, are gene poor and carry genes that are expressed at low levels. We show that ridges are in general less condensed, more irregularly shaped, and located more closely to the nuclear center than antiridges. Six human cell lines that display different gene expression patterns and karyotypes share these structural parameters of chromatin. This shows that the chromatin structures of these two types of genomic domains are largely independent of tissue-specific variations in gene expression and differentiation state. Moreover, we show that there is remarkably little intermingling of chromatin from different parts of the same chromosome in a chromosome territory, neither from adjacent nor from distant parts. This suggests that the chromosomal fiber has a compact structure that sterically suppresses intermingling. Together, our results reveal novel general aspects of 3D chromosome architecture that are related to genome structure and function. |
| Databáze: | OpenAIRE |
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