Combined use of microwave ablation and cell immunotherapy induces nonspecific immunity of hepatocellular carcinoma model mice
| Autor: | Xinwei Han, Jianzhuang Ren, Guo-Hao Huang, Ju Shuguang, Zhang Qinghui, Wang Manzhou, Xuhua Duan |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Carcinoma Hepatocellular Combination therapy medicine.medical_treatment Programmed Cell Death 1 Receptor Combined use Cell Mice Nude Biology Mice 03 medical and health sciences 0302 clinical medicine medicine Animals CTLA-4 Antigen Microwaves Immune Checkpoint Inhibitors Molecular Biology Mice Inbred BALB C Innate immune system Liver Neoplasms Microwave ablation Immunity Cell Biology Immunotherapy Th1 Cells Radiofrequency Therapy medicine.disease Combined Modality Therapy Tumor Burden Survival Rate Disease Models Animal Treatment Outcome 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research Cytokines Research Paper Signal Transduction T-Lymphocytes Cytotoxic Developmental Biology |
| Zdroj: | Cell Cycle |
| ISSN: | 1551-4005 1538-4101 |
| Popis: | Microwave ablation (MWA) has been widely used in the treatment of solid tumors. Studies have been less conducted on the efficacy of MWA used with cell immunotherapy in treating hepatocellular carcinoma (HCC). The current study aimed at exploring the efficacy of MWA in combination with cell immunotherapy in treating HCC. Hepa1-6 HCC mice were treated by MWA, blockade, or the combined therapy (MWA used with blockade), or left untreated. Survival rates of the mice were plotted by Kaplan-Meier Curve, followed by log-rank test. 25 days after the operation, surviving mice were monitored for tumor recurrence, and tumor volumes were calculated every 5 days. Immunohistochemistry and flow cytometry were performed to detect the numbers of CD4(+) and CD8(+) cells in the tumors and spleens of mice. The expressions of related cytokines were detected and measured by ELISPOT and ELISA. The results showed that MWA combined with anti-PD-1/anti-CTLA-4 not only increased the survival time, protected the mice against tumor recurrence, but also enhanced the intratumoral infiltration of cytotoxic T lymphocyte and systemic T-cell immune responses induced by MWA through activation of synergistically specific antitumor immunity. In addition, the combined therapy increased T-helper 1 cell (Th1-type) cytokines, but reduced Th2-type cytokines, resulting in the polarization of Th1 cells. T-cell immune responses of HCC cells were activated by MWA. In addition, the combined therapy of MWA and anti-PD-1/anti-CTLA-4 induced Th1-type immune response, and showed specific antitumor immunity. |
| Databáze: | OpenAIRE |
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