No evidence for a relationship between farm or transformation process locations and antibiotic resistance patterns of Pseudomonas population associated with rainbow trout (Oncorhynchus mykiss)
Autor: | Ségolène Calvez, Agnès Bouju-Albert, Hervé Prévost, Gladys Pangga, Nicolas Helsens, Catherine Magras, Kenny Oberlé |
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Rok vydání: | 2022 |
Předmět: |
Veterinary medicine
medicine.drug_class animal diseases Antibiotics Population Pseudomonas fluorescens Microbial Sensitivity Tests Applied Microbiology and Biotechnology law.invention Fish Diseases Antibiotic resistance law Pseudomonas medicine Animals education education.field_of_study biology Drug Resistance Microbial General Medicine biology.organism_classification Transformation (genetics) Gram staining Oncorhynchus mykiss Rainbow trout Biotechnology |
Zdroj: | Journal of Applied Microbiology. 132:1738-1750 |
ISSN: | 1365-2672 1364-5072 |
Popis: | Aims Study the relationship between antibiotic resistance patterns of Pseudomonas isolated from farmed rainbow trout fillets and farm or transformation process locations. Methods and Results Pseudomonas strains were isolated from rainbow trout sampled in two differently located farms and filleted in laboratory or in a processing factory. One hundred and twenty-five isolates were confirmed as belonging to Pseudomonas using CFC selective media, Gram staining, oxidase test and quantitative polymerase chain reaction methods. Fifty-one isolates from separate fish fillets were further identified using MALDI-TOF mass spectrometry, and the minimal inhibitory concentrations (MIC) of 11 antibiotics were also determined by microdilution method. Most of the isolates belonged to the Pseudomonas fluorescens group (94.1%), and no relationship was established between antibiotic resistance patterns and sampling locations (farms or filleting areas). Multiple resistance isolates with high MIC values (from 64 µg ml−1 to more than 1024 µg ml−1) were identified. Conclusions Antibiotic resistance patterns found in Pseudomonas isolates were not influenced by farms or transformation process locations. Seven isolates were found highly resistant to four different antibiotic classes. Significance and Impact of the Study This study does not provide evidence of a relationship between farm or transformation process locations on antibiotic resistance patterns of Pseudomonas population. |
Databáze: | OpenAIRE |
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