Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles
| Autor: | Chengyu Lu, Hongmei Lu, Jianxin Shi, Zhi Chen, Xiaoliu Liu, Dudu Wu, Kangrui Yuan, Kun Liu, Wei Liu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
biology
Biocompatibility Chemistry ZnO nanoparticles General Chemistry biology.organism_classification Antimicrobial medicine.disease_cause chemistry.chemical_compound enzyme antibacterial activity Staphylococcus aureus medicine Agar diffusion test Lysozyme Fourier transform infrared spectroscopy Antibacterial activity QD1-999 lysozyme Bacteria nanomaterials Nuclear chemistry Original Research |
| Zdroj: | Frontiers in Chemistry Frontiers in Chemistry, Vol 9 (2021) |
| ISSN: | 2296-2646 |
| Popis: | The lysozyme-modified nanoparticles (LY@ZnO NPs) were synthesized by the reduction–oxidation method, and the morphology and structure of LY@ZnO were analyzed by Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (XRD), scanning electron microsclope (SEM), and particle size analysis. The antibacterial effects of LY@ZnO against Escherichia coli (E. coli, Gram-negative bacteria) and Staphylococcus aureus (S. aureus, Gram-positive bacteria) were discussed by measuring the zone of inhibition (ZOI) and growth inhibition. The antimicrobial experiments showed that the LY@ZnO NPs possessed better antibacterial activity than ZnO. Besides, the antibacterial mechanism of LY@ZnO was also investigated, which was attributed to the generation of reactive oxygen species (ROS). Furthermore, the toxicities of LY@ZnO in vivo and in vitro were discussed by the cell counting kit-8 method and animal experiments, showing that LY@ZnO possessed excellent biocompatibility. Finally, the therapeutic effect of LY@ZnO on a rat skin infection model caused by methicillin-resistant Staphylococcus aureus (MRSA) was also studied, which exhibited good anti-infective activity. Our findings showed that LY@ZnO possessed remarkable antibacterial ability due to its excellent membrane permeability and small particle size. Besides, LY@ZnO also exhibited certain stability and great safety, which showed tremendous prospects for microbial infection in patients. It would also be helpful for a better understanding of the enzyme-modified nanomaterials against bacteria. |
| Databáze: | OpenAIRE |
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