Versatility of a localized surface plasmon resonance-based gold nanoparticle-alloyed quantum dot nanobiosensor for immunofluorescence detection of viruses

Autor: Tomoyuki Tanaka, Tatsuya Kato, Oluwasesan Adegoke, Tetsuro Suzuki, Tian-Cheng Li, Naoto Takahashi, Noritoshi Kitamoto, Enoch Y. Park, Kenshin Takemura
Rok vydání: 2017
Předmět:
Gold nanoparticle
Biomedical Engineering
Biophysics
Metal Nanoparticles
Nanoparticle
Hemagglutinin Glycoproteins
Influenza Virus
Nanotechnology
Biosensing Techniques
02 engineering and technology
01 natural sciences
Virus
Influenza A Virus
H1N1 Subtype
Limit of Detection
Influenza
Human
Quantum Dots
Electrochemistry
Humans
Cysteine
Surface plasmon resonance
Detection limit
Immunofluorescence detection
Chemistry
Influenza A Virus
H3N2 Subtype
Norovirus
010401 analytical chemistry
technology
industry
and agriculture
virus diseases
Alloyed quantum dot
General Medicine
Surface Plasmon Resonance
Localized surface plasmon resonance
021001 nanoscience & nanotechnology
Ligand (biochemistry)
Fluorescence
0104 chemical sciences
Quantum dot
Gold
Influenza virus
0210 nano-technology
Biosensor
Biotechnology
Zdroj: Biosensors and Bioelectronics. 89:998-1005
ISSN: 0956-5663
DOI: 10.1016/j.bios.2016.10.045
Popis: Flu infection, caused by the influenza virus, constitutes a serious threat to human lives worldwide. A rapid, sensitive and specific diagnosis is urgently needed for point-of-care treatment and to control the rapid spread of this disease. In this study, an ultrasensitive, rapid and specific localized surface plasmon resonance (LSPR)-induced immunofluorescence nanobiosensor has been developed for the influenza virus based on a gold nanoparticle (AuNP)-induced quantum dot (QD) fluorescence signal. Alloyed quaternary CdSeTeS QDs were synthesized via the hot-injection organometallic route and were subsequently capped with l-cysteine via a ligand exchange reaction. AuNPs were synthesized in HEPES buffer and thiolated with l-cysteine. The concept of the biosensor involves the conjugation of anti-neuraminidase (NA) antibody (anti-NA Ab) to thiolated AuNPs and the conjugation of anti-hemagglutinin (HA) antibody (anti-HA Ab) to alloyed quaternary l-cysteine-capped CdSeTeS QDs. Interaction of the antigens displaying on the surface of the influenza virus target with anti-NA Ab-conjugated AuNPs and anti-HA Ab-conjugated QDs induces an LSPR signal from adjacent AuNPs to trigger fluorescence-enhancement changes in the QDs in proportion to the concentration of the target virus. The detection limit for influenza H1N1 virus was 0.03pg/mL in deionized water and 0.4pg/mL in human serum; while, for the clinically isolated H3N2, the detection limit was 10PFU/mL. The detection of influenza virus H1N1 was accomplished with high sensitivity. The versatility of the biosensor was demonstrated for the detection of clinically isolated influenza virus H3N2 and norovirus-like particles (NoV-LPs).
Databáze: OpenAIRE
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