Novel European free-living, non-diazotrophic Bradyrhizobium isolates from contrasting soils that lack nodulation and nitrogen fixation genes – a genome comparison
Autor: | Robert King, Martin J. Woodward, Liz J. Shaw, Ian M. Clark, Frances Patricia Jones, Penny R. Hirsch |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Root nodule 030106 microbiology Plant Root Nodulation Genome Bradyrhizobium Article 03 medical and health sciences Genome Size Nitrogen Fixation Botany Genome size Phylogeny Soil Microbiology Multidisciplinary biology food and beverages Molecular Sequence Annotation Sequence Analysis DNA 15. Life on land biology.organism_classification Europe 030104 developmental biology Nitrogen fixation Diazotroph Root Nodules Plant Soil microbiology Genome Bacterial |
Zdroj: | Scientific Reports |
ISSN: | 2045-2322 |
Popis: | The slow-growing genus Bradyrhizobium is biologically important in soils, with different representatives found to perform a range of biochemical functions including photosynthesis, induction of root nodules and symbiotic nitrogen fixation and denitrification. Consequently, the role of the genus in soil ecology and biogeochemical transformations is of agricultural and environmental significance. Some isolates of Bradyrhizobium have been shown to be non-symbiotic and do not possess the ability to form nodules. Here we present the genome and gene annotations of two such free-living Bradyrhizobium isolates, named G22 and BF49, from soils with differing long-term management regimes (grassland and bare fallow respectively) in addition to carbon metabolism analysis. These Bradyrhizobium isolates are the first to be isolated and sequenced from European soil and are the first free-living Bradyrhizobium isolates, lacking both nodulation and nitrogen fixation genes, to have their genomes sequenced and assembled from cultured samples. The G22 and BF49 genomes are distinctly different with respect to size and number of genes; the grassland isolate also contains a plasmid. There are also a number of functional differences between these isolates and other published genomes, suggesting that this ubiquitous genus is extremely heterogeneous and has roles within the community not including symbiotic nitrogen fixation. |
Databáze: | OpenAIRE |
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