A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes
Autor: | Alejandro Cisneros, J.G. Mendoza-Alvarez, Victor Altuzar, Joel Navarrete-Espinosa, María de Lourdes Muñoz, F. Sánchez-Sinencio, Juan Burgueño, Jorge P. Martínez-Muñoz, Gerardo Pérez-Ramírez, Alvaro Diaz-Badillo |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Serotype
Microarray viruses Biosensing Techniques Biology Dengue virus Serogroup medicine.disease_cause lcsh:Chemical technology Sensitivity and Specificity Biochemistry Article Analytical Chemistry Microbiology Dengue fever Dengue chemistry.chemical_compound Aedes medicine A-DNA lcsh:TP1-1185 Electrical and Electronic Engineering humans Instrumentation microarrays Oligonucleotide Array Sequence Analysis dengue virus Reproducibility of Results virus diseases Equipment Design biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Virology Atomic and Molecular Physics and Optics Equipment Failure Analysis chemistry DNA Viral DNA microarray DNA |
Zdroj: | Sensors, Vol 14, Iss 5, Pp 7580-7601 (2014) Sensors; Volume 14; Issue 5; Pages: 7580-7601 Sensors (Basel, Switzerland) |
ISSN: | 1424-8220 |
Popis: | Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples. |
Databáze: | OpenAIRE |
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