Ma Orthologous Genes in Prunus spp. Shed Light on a Noteworthy NBS-LRR Cluster Conferring Differential Resistance to Root-Knot Nematodes
| Autor: | Caroline Callot, Cyril Van Ghelder, Daniel Esmenjaud, Emeric Dubois, H. Duval, Marianne Mazier |
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| Přispěvatelé: | Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), Génétique et Amélioration des Fruits et Légumes (GAFL), C2011-01, INRA BAP department IVD2 IVD3 |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine mécanisme de résistance meloidogyne Plant Science lcsh:Plant culture Biology structure nodulaire 01 natural sciences Genome almond 03 medical and health sciences Exon Prunus Gene mapping resistance gene [SDV.BV]Life Sciences [q-bio]/Vegetal Biology lcsh:SB1-1110 gène orthologue Gene nématode Genetics Bacterial artificial chromosome Vegetal Biology Gene map Meloidogyne nucleotide-binding site - leucine-rich repeat (NBS-LRR) root-knot nematodes (RKNs) amande food and beverages gène de résistance prunus 030104 developmental biology site de liaison protéique séquence riche en leucine Biologie végétale 010606 plant biology & botany Reference genome |
| Zdroj: | Frontiers in Plant Science (9), . (2018) Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2018, 9, ⟨10.3389/fpls.2018.01269⟩ Frontiers in Plant Science, Vol 9 (2018) |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2018.01269 |
| Popis: | International audience; Root-knot nematodes (RKNs) are considerable polyphagous pests that severely challenge plants worldwide and especially perennials. The specific genetic resistance of plants mainly relies on the NBS-LRR genes that are pivotal factors for pathogens control. In Prunus spp., the Ma plum and RMja almond genes possess different spectra for resistance to RKNs. While previous works based on the Ma gene allowed to clone it and to decipher its peculiar TIR-NBS-LRR (TNL) structure, we only knew that the RMja gene mapped on the same chromosome as Ma. We carried out a high-resolution mapping using an almond segregating F2 progeny of 1448 seedlings from resistant (R) and susceptible (S) parental accessions, to locate precisely RMja on the peach genome, the reference sequence for Prunus species. We showed that the RMja gene maps in the Ma resistance cluster and that the Ma ortholog is the best candidate for RMja. This co-localization is a crucial step that opens the way to unravel the molecular determinants involved in the resistance to RKNs. Then we sequenced both almond parental NGS genomes and aligned them onto the RKN susceptible reference peach genome. We produced a BAC library of the R parental accession and, from two overlapping BAC clones, we obtained a 336-kb sequence encompassing the RMja candidate region. Thus, we could benefit from three Ma orthologous regions to investigate their sequence polymorphism, respectively, within plum (complete R spectrum), almond (incomplete R spectrum) and peach (null R spectrum). We showed that the Ma TNL cluster has evolved orthologs with a unique conserved structure comprised of five repeated postLRR (PL) domains, which contain most polymorphism. In addition to support the Ma and RMja orthologous relationship, our results suggest that the polymorphism contained in the PL sequences might underlie differential resistance interactions with RKNs and an original immune mechanism in woody perennials. Besides, our study illustrates how PL exon duplications and losses shape TNL structure and give rise to atypical PL domain repeats of yet unknown role. |
| Databáze: | OpenAIRE |
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