Transient B-cell depletion with anti-CD20 in combination with proinsulin DNA vaccine or oral insulin: immunologic effects and efficacy in NOD mice
| Autor: | Lawrence Steinman, Sowbarnika Sachithanantham, Mark A. Atkinson, Marilyne Coulombe, Hideki Garren, Ghanashyam Sarikonda, Mario R. Ehlers, Yulia Manenkova, Teodora Staeva, Philip Bernstein, Gerald T. Nepom, Teresa Rodriguez Calvo, Darius A. Schneider, Clive Wasserfall, Ronald G. Gill, Tinalyn Kupfer, Kevan C. Herold, Andrew C. Chan, Philippe P. Pagni, Matthias von Herrath, Laura Straub, Amanda L. Posgai |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
CD4-Positive T-Lymphocytes
Anatomy and Physiology B Cells Mouse endocrine system diseases medicine.medical_treatment Administration Oral lcsh:Medicine Autoimmunity Mice 0302 clinical medicine Endocrinology Insulin Signaling Cascade Mice Inbred NOD Molecular Cell Biology Vaccines DNA Insulin lcsh:Science NOD mice Proinsulin 0303 health sciences B-Lymphocytes Multidisciplinary biology T Cells Antibodies Monoclonal Animal Models Signaling Cascades 3. Good health Medicine Drug Therapy Combination Female Immunotherapy Antibody Research Article Signal Transduction medicine.medical_specialty endocrine system Combination therapy Immune Cells Immunology Endocrine System Immune Suppression Immunomodulation 03 medical and health sciences Model Organisms Antigen Diabetes mellitus Internal medicine medicine Immune Tolerance Animals Biology Pancreas 030304 developmental biology Diabetic Endocrinology Type 1 diabetes Endocrine Physiology business.industry lcsh:R Immunity Immunoregulation Diabetes Mellitus Type 1 medicine.disease Antigens CD20 Mice Inbred C57BL Diabetes Mellitus Type 1 Hyperglycemia biology.protein lcsh:Q Interleukin-4 Lymph Nodes business Spleen 030215 immunology |
| Zdroj: | PLoS ONE, Vol 8, Iss 2, p e54712 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | A recent type 1 diabetes (T1D) clinical trial of rituximab (a B cell-depleting anti-CD20 antibody) achieved some therapeutic benefit in preserving C-peptide for a period of approximately nine months in patients with recently diagnosed diabetes. Our previous data in the NOD mouse demonstrated that co-administration of antigen (insulin) with anti-CD3 antibody (a T cell-directed immunomodulator) offers better protection than either entity alone, indicating that novel combination therapies that include a T1D-related autoantigen are possible. To accelerate the identification and development of novel combination therapies that can be advanced into the clinic, we have evaluated the combination of a mouse anti-CD20 antibody with either oral insulin or a proinsulin-expressing DNA vaccine. Anti-CD20 alone, given once or on 4 consecutive days, produced transient B cell depletion but did not prevent or reverse T1D in the NOD mouse. Oral insulin alone (twice weekly for 6 weeks) was also ineffective, while proinsulin DNA (weekly for up to 12 weeks) showed a trend toward modest efficacy. Combination of anti-CD20 with oral insulin was ineffective in reversing diabetes in NOD mice whose glycemia was controlled with SC insulin pellets; these experiments were performed in three independent labs. Combination of anti-CD20 with proinsulin DNA was also ineffective in diabetes reversal, but did show modest efficacy in diabetes prevention (p = 0.04). In the prevention studies, anti-CD20 plus proinsulin resulted in modest increases in Tregs in pancreatic lymph nodes and elevated levels of proinsulin-specific CD4+ T-cells that produced IL-4. Thus, combination therapy with anti-CD20 and either oral insulin or proinsulin does not protect hyperglycemic NOD mice, but the combination with proinsulin offers limited efficacy in T1D prevention, potentially by augmentation of proinsulin-specific IL-4 production. |
| Databáze: | OpenAIRE |
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