Upregulation of endothelial nitric oxide synthase in rat aorta after ingestion of fish oil-rich diet
| Autor: | Diego López, Xavier Orta, P. Puig-Parellada, M. Teresa Mitjavila, Mireia Farriol, Kelly Casós, M. Puy Sáiz |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Nitric Oxide Synthase Type III Endothelium Physiology Arginine Nitric Oxide Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Fish Oils Dietary Fats Unsaturated Downregulation and upregulation Superoxides Physiology (medical) Internal medicine medicine.artery medicine Animals Ingestion RNA Messenger Cyclic GMP Aorta Dose-Response Relationship Drug biology Chemistry Electron Spin Resonance Spectroscopy Fish oil Rats Up-Regulation Nitric oxide synthase medicine.anatomical_structure Endocrinology Biochemistry biology.protein Citrulline Nitric Oxide Synthase Oxidoreductases Cardiology and Cardiovascular Medicine Blood vessel |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 287:H567-H572 |
| ISSN: | 1522-1539 0363-6135 |
| Popis: | A previous study with aortic segments isolated from rats fed a fish oil-rich diet indicated an increase in acetylcholine-induced nitric oxide (.NO)-mediated relaxation. However, it remained to be elucidated whether a fish oil-rich diet affects the vascular activity per se and the point of the.NO-cGMP pathway at which fish oil acts. For this purpose, two groups of Sprague-Dawley rats were fed a semipurified diet containing 5% lipids, either corn oil (CO) or menhaden oil (MO), for 8 wk. We studied the mRNA and protein levels of endothelial NO synthase (eNOS) and NOS activity. The bioavailability of vascular.NO was assessed directly by electron spin resonance spectroscopy. The levels of cGMP, l-arginine, and l-citrulline were also evaluated in homogenates. Superoxide anion (O(2)(-).) production and related antioxidant activities were also studied in aortic segments. The aortic content of eNOS mRNA was increased in rats fed the MO-rich diet. This resulted in increases in both eNOS protein levels (70% relative to the rats fed the CO-rich diet) and NOS activity (102%);.NO production increased by 90%, cGMP levels increased by 100%, and l-arginine decreased by 30%. No change in aortic O(2)(-). production was caused by dietary MO. The upregulation of the eNOS-cGMP pathway induced by dietary MO may contribute to the maintenance of vascular homeostasis and explain its beneficial effect in the prevention of arterial diseases. |
| Databáze: | OpenAIRE |
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