Physicochemical properties of PEG-grafted liposomes
| Autor: | Masaharu Ueno, Supaporn Sriwongsitanont |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Chemical Phenomena
Polyethylene glycol Micelle Polyethylene Glycols chemistry.chemical_compound Drug Discovery PEG ratio Pharmaceutic Aids Freeze Fracturing Particle Size Phospholipids Liposome Drug Carriers Chromatography Chemistry Physical Bilayer Vesicle Phosphatidylethanolamines technology industry and agriculture General Chemistry General Medicine Fluoresceins Calcein Microscopy Electron Cholesterol chemistry Liposomes Chromatography Gel lipids (amino acids peptides and proteins) Drug carrier Dimyristoylphosphatidylcholine |
| Zdroj: | Chemicalpharmaceutical bulletin. 50(9) |
| ISSN: | 0009-2363 |
| Popis: | Egg phosphatidylcholine (EggPC) or dimyristoylphosphatidylcholine (DMPC) liposomes containing polyethylene glycol (PEG)-lipids covering a range of 0-30 mol% have been prepared by Extrusion method. The physicochemical properties including size evolution and calcein permeation were evaluated to investigate the effect of PEG-lipids on bilayer structure. The results from quasielasetic light scattering (QELS), freeze-fracture microscopy, and gel exclusion chromatography revealed that presence of low concentration of PEG-lipid results in decreasing of vesicle size and further increase in the PEG-lipid concentrations lead to a transition from the lamellar membranes to micelles. The permeability for calcein increased with increase in concentration of distearoylphosphatidylethanolamine (DSPE)-PEG. On the other hand, the permeability decreased with low amount of cholesterol-PEG (blow 20% cholesterol-PEG) and increased with high amount of it. The maximum concentration of PEG-lipid that may be incorporated without alteration of the liposome structure depends on the composition of the bilayer. The concentration of DSPE-PEG2000 incorporated into vesicles without damaging vesicle structures were |
| Databáze: | OpenAIRE |
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