Micropatterns of an Extracellular Matrix Protein with Defined Information Content
| Autor: | Jeffrey L. Werbin, Jan H. Hoh, William F. Heinz, Lewis H. Romer |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Swiss 3T3 Cells
Extracellular Matrix Proteins biology Surface Properties Computer science Information Theory Nanotechnology Surfaces and Interfaces Condensed Matter Physics Information theory Models Biological Fibronectins Fibronectin Extracellular matrix Mice Microscopy Fluorescence Electrochemistry biology.protein Animals Entropy (information theory) General Materials Science Biological system Spectroscopy Signal Transduction |
| Zdroj: | Langmuir. 23:10883-10886 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la701605s |
| Popis: | One powerful approach to understanding how cells process spatially variant signals is based on using micropatterned substrates to control the distribution of signaling molecules. However, quantifying spatially complex signals requires an appropriate metric. Here we propose that the Shannon information theory formalism provides a robust and useful way to quantify the organization of proteins in micropatterned systems. To demonstrate the use of informational entropy as a metric, we produced patterns of lines of fibronectin with varying information content. Fibroblasts grown on these patterns were sensitive to very small changes in informational entropy (6.6 bits), and the responses depended on the scale of the pattern. |
| Databáze: | OpenAIRE |
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