RGS4 RNA secondary structure mediates staufen2 RNP assembly in neurons
| Autor: | Rico Schieweck, Sandra M. Fernández-Moya, Flora C.Y. Lee, Janina Ehses, Max Harner, Karl E. Bauer, Nicholas M. Luscombe, Michael A. Kiebler, Christin Illig, Jernej Ule, Anob M. Chakrabarti |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Untranslated region
RNA localization QH301-705.5 RNA-binding protein Gene Expression Biochemistry & Proteomics Staufen2 neuronal RNA granule RNP assembly in vivo RNA binding Catalysis Article Nucleic acid secondary structure Inorganic Chemistry Turn (biochemistry) RGS4 03 medical and health sciences 0302 clinical medicine Ecology Evolution & Ethology Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy 030304 developmental biology Computational & Systems Biology 0303 health sciences biology Chemistry FOS: Clinical medicine Stem Cells Organic Chemistry Neurosciences RNA Translation (biology) General Medicine Tumour Biology Computer Science Applications Cell biology biology.protein Genetics & Genomics 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 13021, p 13021 (2021) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 13021 |
| DOI: | 10.25418/crick.17151815 |
| Popis: | RNA-binding proteins (RBPs) act as posttranscriptional regulators controlling the fate of target mRNAs. Unraveling how RNAs are recognized by RBPs and in turn are assembled into neuronal RNA granules is therefore key to understanding the underlying mechanism. While RNA sequence elements have been extensively characterized, the functional impact of RNA secondary structures is only recently being explored. Here, we show that Staufen2 binds complex, long-ranged RNA hairpins in the 3′-untranslated region (UTR) of its targets. These structures are involved in the assembly of Staufen2 into RNA granules. Furthermore, we provide direct evidence that a defined Rgs4 RNA duplex regulates Staufen2-dependent RNA localization to distal dendrites. Importantly, disrupting the RNA hairpin impairs the observed effects. Finally, we show that these secondary structures differently affect protein expression in neurons. In conclusion, our data reveal the importance of RNA secondary structure in regulating RNA granule assembly, localization and eventually translation. It is therefore tempting to speculate that secondary structures represent an important code for cells to control the intracellular fate of their mRNAs. |
| Databáze: | OpenAIRE |
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