Re-expression of miR-199a suppresses renal cancer cell proliferation and survival by targeting GSK-3β
| Autor: | Tomoyuki Kato, Sei Naito, Vladimir Bilim, Kaori Yuuki, Masaaki Tsukigi, Teiichi Motoyama, Akira Nagaoka, Andrey Ugolkov, Yoshihiko Tomita |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Cancer Research Cell Survival medicine.medical_treatment Blotting Western Down-Regulation macromolecular substances Biology urologic and male genital diseases Targeted therapy Glycogen Synthase Kinase 3 Downregulation and upregulation Renal cell carcinoma Cell Line Tumor medicine Humans Carcinoma Renal Cell Aged Cell Proliferation Regulation of gene expression Aged 80 and over Cell growth Middle Aged medicine.disease Molecular biology Immunohistochemistry female genital diseases and pregnancy complications Gene Expression Regulation Neoplastic MicroRNAs Oncology Cell culture Apoptosis Cancer cell Cancer research Female |
| Zdroj: | Cancer letters. 315(2) |
| ISSN: | 1872-7980 |
| Popis: | Recently, we have identified GSK-3 as a new therapeutic target in renal cell cancer (RCC). miR-199a could potentially downregulate GSK-3β expression. Here, we found a decreased miR-199a expression in 59% (32 of 54) of RCCs and it was correlated with higher tumor stage (p < 0.05) and nuclear overexpression of GSK-3β (p < 0.05). We show that re-expression of miR-199a downregulates GSK-3β and suppresses cancer cell growth. Our results demonstrate low miR-199a expression as a feature of advanced RCCs, identify miR-199a as a negative regulator of GSK-3β, and suggest re-expression of pre-miR-199a as a new potential treatment of RCC. |
| Databáze: | OpenAIRE |
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