Oral cancer diagnostics based on infrared spectral markers and wax physisorption kinetics
| Autor: | Dar-Bin Shieh, Wei Fan Chiang, Tung Yiu Wong, Yao Chang Lee, Pei Yu Huang, Li Fang Chiu, Sheng Hsiang Lin |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Keratinocytes
In situ Primary Cell Culture Analytical chemistry Xylenes Biochemistry Beeswax Analytical Chemistry Malignant transformation Physisorption Paraffin wax Cell Line Tumor Spectroscopy Fourier Transform Infrared Humans Fourier transform infrared spectroscopy Wax Chromatography Chemistry Discriminant Analysis Kinetics Cell Transformation Neoplastic Early Diagnosis Waxes visual_art Cancer cell visual_art.visual_art_medium Mouth Neoplasms |
| Zdroj: | Analytical and Bioanalytical Chemistry. 405:1995-2007 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-012-6625-z |
| Popis: | Infrared microspectroscopy is an emerging approach for disease analysis owing to its capability for in situ chemical characterization of pathological processes. Synchrotron-based infrared microspectroscopy (SR-IMS) provides ultra-high spatial resolution for profiling biochemical events associated with disease progression. Spectral alterations were observed in cultured oral cells derived from healthy, precancerous, primary, and metastatic cancers. An innovative wax-physisorption-based kinetic FTIR imaging method for the detection of oral precancer and cancer was demonstrated successfully. The approach is based on determining the residual amount of paraffin wax (C(25)H(52)) or beeswax (C(46)H(92)O(2)) on a sample surface after xylene washing. This amount is used as a signpost of the degree of physisorption that altered during malignant transformation. The results of linear discriminant analysis (LDA) of oral cell lines indicated that the methylene (CH(2)) and methyl group (CH(3)) stretching vibrations in the range of 3,000-2,800 cm(-1) have the highest accuracy rate (89.6 %) to discriminate the healthy keratinocytes (NHOK) from cancer cells. The results of wax-physisorption-based FTIR imaging showed a stronger physisorption with beeswax in oral precancerous and cancer cells as compared with that of NHOK, which showed a strong capability with paraffin wax. The infrared kinetic study of oral cavity tissue showed a consistency in the wax physisorption of the cell lines. On the basis of our findings, these results show the potential use of wax-physisorption-based kinetic FTIR imaging for the early screening of oral cancer lesions and the chemical changes during oral carcinogenesis. |
| Databáze: | OpenAIRE |
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