The detection of penicillin insensitivity in Neisseria meningitidis by polymerase chain reaction
| Autor: | J. M. J. Logan, A. F. Maggs, T. H. Pennington, P. E. Carter |
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| Rok vydání: | 1998 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Penicillin binding proteins Penicillin Resistance Neisseria flavescens Microbial Sensitivity Tests Muramoylpentapeptide Carboxypeptidase Neisseria meningitidis medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology Antibiotic resistance Bacterial Proteins law Multienzyme Complexes medicine Humans Penicillin-Binding Proteins Pharmacology (medical) Polymerase chain reaction Antibacterial agent Pharmacology biology biology.organism_classification Virology Penicillin Infectious Diseases Hexosyltransferases Peptidyl Transferases Neisseria Carrier Proteins medicine.drug |
| Zdroj: | The Journal of antimicrobial chemotherapy. 42(3) |
| ISSN: | 0305-7453 |
| Popis: | Strains of penicillin-sensitive and -insensitive Neisseria meningitidis were examined using a range of polymerase chain reaction (PCR) primers directed at the meningococcal penicillin-binding protein 2 gene. DNA from isolates whose penicillin MIC was0.2 mg/L yielded a product of the expected size with all the primers, but many amplification patterns were seen with DNA from isolates whose MIC was above this level. All strains whose MIC was0.25 mg/L failed to produce a product of the expected size with at least one of the primers used. The changes seen in penicillin-insensitive strains were consistent with horizontal gene transfer from Neisseria flavescens in some isolates, although the source for others remains unknown. PCR-based methods for the detection of antibiotic resistance are becoming increasingly important with the expanding use of molecular techniques for bacteriological diagnosis. |
| Databáze: | OpenAIRE |
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