Recurrent water deficit causes alterations in the profile of redox proteins in citrus plants
| Autor: | Abelmon da Silva Gesteira, Claudia Fortes Ferreira, Diana Matos Neves, Milena Santos Dória, Walter dos Santos Soares Filho, Dayse Drielly Souza Santana-Vieira, Fabienne Micheli, Maurício Antônio Coelho Filho, Marcio Gilberto Cardoso Costa, Luciano Freschi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Citrus Physiology F60 - Physiologie et biochimie végétale Carbohydrates Plant Science medicine.disease_cause 01 natural sciences Acclimatization Redox F30 - Génétique et amélioration des plantes 03 medical and health sciences chemistry.chemical_compound Genetics medicine Electrophoresis Gel Two-Dimensional Glyceraldehyde 3-phosphate dehydrogenase Plant Proteins chemistry.chemical_classification Principal Component Analysis Reactive oxygen species Staining and Labeling biology Water food and beverages Fructose Droughts Plant Leaves Phenotype 030104 developmental biology chemistry Biochemistry Catalase biology.protein Rootstock Oxidation-Reduction Oxidative stress 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2018.09.035 |
| Popis: | Plant acclimation to recurrent stress involves profound alterations in multiple genetic, metabolic and physiological processes. Stressful conditions usually implicate imbalance in reactive oxygen species (ROS) production and removal rates, which may lead to oxidative stress. However, the primary cellular targets of oxidative stress and their relevance in plant acclimation to abiotic stresses remains poorly characterized. By comparing redox proteomic and sugar profiles in citrus Valencia (VO) scions grafted onto two rootstocks with different soil water extraction capacities - Rangpur Lime (RL) and Sunki Maravilha (SM) - here we demonstrate that both ROS-mediated post-translational protein modification and changes in sugar composition are associated with acclimation to recurrent drought in citrus. The redox proteomic analysis of the distinct scion/rootstock combinations exposed to one (WD1), two (WD2) or three (WD3) water deficit episodes revealed a total of 32 and 55 redox protein spots present in VO/RL and VO/SM plants, respectively. Mass spectrometry analysis of these protein spots revealed essential targets of ROS-mediated posttranslational protein modification in citrus plants challenged by recurrent drought. The oxidation of cysteine thiol groups into glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was shown to increase in WD3 samples of the VO/RL combination, whereas the opposite was observed for the VO/SM combination. Similarly, recurrent drought promoted the oxidation of catalase thiol groups in VO/SM, but not in VO/RL. Carbohydrate profiling revealed that glucose, fructose and galactose may also contribute to the phenotypic differences observed between the citrus genotypes exposed to drought. These findings reveal for the first time that recurrent drought differentially affects the profile of redox proteomics of citrus, suggesting that this alteration may be part of the stress memory in perennial plants. |
| Databáze: | OpenAIRE |
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