Salmonella/mammalian microsome assay with tetranitromethane and 3-nitro-L-tyrosine
| Autor: | U. Friederich, M. Ganss, E. Fürer, F.E. Würgler |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Salmonella typhimurium
endocrine system Nitro compound Mutagen In Vitro Techniques medicine.disease_cause Ames test chemistry.chemical_compound Microsomes medicine Animals Tyrosine Cells Cultured chemistry.chemical_classification Dose-Response Relationship Drug Chemistry Mutagenicity Tests fungi food and beverages General Medicine Tetranitromethane Rats Biochemistry Nitrosation Microsome Nitro Methane Mutagens |
| Zdroj: | Mutation research. 244(1) |
| ISSN: | 0027-5107 |
| Popis: | The nitrosating agent tetranitromethane (TNM) and the nitrosation product 3-nitro- l -tyrosine (NT) were tested for mutagenic activity in the Salmonella/ mammalian microsome assay. TNM showed strong genotoxic activity: it was mutagenic in all tester strains used (TA97, TA98, TA100, and TA102). Them maximum mutagenic activity was reached between 16 and 32 μg/plate using the standard plate test; higher amounts led to distinct bactericidal effects. The mutagenicity was independent of an in vitro activation system. In the preincubation assay an increased bactericidal effect was observed. In contrast to TNM, NT, the nitrosation product, was non-mutagenic and non-toxic in the standard plate test and with the preincubation method up to 5000 μg/plate with and without S9 mix and with all tester strains used. Although TNM is a strong direct-acting mutagen, its nitrosating effect on proteins does lead to non-genotoxic nitro products of tyrosine in proteins. |
| Databáze: | OpenAIRE |
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