New advances in production and functional folding of G-protein-coupled receptors
| Autor: | Jean-Louis Banères, Bernard Mouillac, Jean-Luc Popot |
|---|---|
| Přispěvatelé: | Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Physico-chimie moléculaire des membranes biologiques (PCMMB), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Mouillac, Bernard |
| Rok vydání: | 2011 |
| Předmět: |
Protein Folding
MESH: Protein Folding Bioengineering MESH: Receptors G-Protein-Coupled Computational biology Biology Inclusion bodies Receptors G-Protein-Coupled MESH: Recombinant Proteins 03 medical and health sciences Escherichia coli Integral membrane protein 030304 developmental biology G protein-coupled receptor Inclusion Bodies 0303 health sciences MESH: Escherichia coli 030302 biochemistry & molecular biology [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences MESH: Inclusion Bodies Recombinant Proteins [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences Folding (chemistry) Membrane protein Biochemistry MESH: Biotechnology Protein folding Biotechnology |
| Zdroj: | Trends in Biotechnology Trends in Biotechnology, Elsevier, 2011, 29 (7), pp.314-22. ⟨10.1016/j.tibtech.2011.03.002⟩ Trends in Biotechnology, 2011, 29 (7), pp.314-22. ⟨10.1016/j.tibtech.2011.03.002⟩ |
| ISSN: | 0167-7799 |
| DOI: | 10.1016/j.tibtech.2011.03.002 |
| Popis: | International audience; G-protein-coupled receptors (GPCRs), the largest family of integral membrane proteins, participate in the regulation of many physiological functions and are the targets of approximately 30% of currently marketed drugs. However, knowledge of the structural and molecular bases of GPCR functions remains limited owing to difficulties related to their overexpression, purification and stabilization. The development of new strategies aimed at obtaining large amounts of functional GPCRs is therefore crucial. Here, we review the most recent advances in the production and functional folding of GPCRs from Escherichia coli inclusion bodies. Major breakthroughs open exciting perspectives for structural and dynamic investigations of GPCRs. In particular, combining targeting to bacterial inclusion bodies with amphipol-assisted folding is emerging as a highly powerful strategy. |
| Databáze: | OpenAIRE |
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