Shape-controlled high cell-density microcapsules by electrodeposition
| Autor: | Zeyang Liu, Masaru Takeuchi, Qiang Huang, Toshio Fukuda, Masahiro Nakajima, Yasuhisa Hasegawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Alginates Cell Culture Techniques Biomedical Engineering Capsules Nanotechnology Core (manufacturing) 02 engineering and technology Biochemistry Cell Line Biomaterials 03 medical and health sciences chemistry.chemical_compound Glucuronic Acid Tissue engineering Animals Polylysine Cell encapsulation Molecular Biology Aqueous solution Hexuronic Acids Electrochemical Techniques General Medicine Cells Immobilized 021001 nanoscience & nanotechnology Glucuronic acid Rats 030104 developmental biology Membrane chemistry Electrode 0210 nano-technology Biotechnology |
| Zdroj: | Acta Biomaterialia. 37:93-100 |
| ISSN: | 1742-7061 |
| Popis: | Cell encapsulation within alginate-poly- l -lysine (PLL) microcapsules has been developed to provide a miniaturized three-dimensional (3D) microenvironment with an aqueous core while promoting development of encapsulated cells into high cell-density structures. In this paper, a novel method for fabricating shape-controlled alginate-PLL microcapsules to construct 3D cell structures based on electrodeposition method is provided. Two-dimensional Ca-alginate cell-laden gel membranes were electrodeposited onto a micro-patterned electrode and further detached from the electrode. The PLL was coated onto the gel structures to form alginate-PLL complex as an outer shell and sodium citric solution was utilized to melt the internal alginate to achieve miniaturized 3D microcapsules (sphere, cuboid, and rod shape). By this proposed method, rat liver cells (RLC-18) formed multi-cellular aggregates with high cell-density after cultivation for 2 weeks. Statement of Significance The use of alginate-poly- l -lysine (PLL) microcapsules has shown great potential in fabricating 3D cell structures with high cell density. Despite their success related to their ability to provide a miniaturized microenvironment with an aqueous core, alginate-PLL microcapsules has drawback such as a limited shape-control ability. Because of the mechanism of Ca-induced alginate gel formation, it is still difficult to precisely control the gelation process to produce alginate-PLL microcapsules with specific shape. The present study provides an electrodeposition-based method to generate shape-controlled microcapsules for 3D cell structures. Sphere, cuboid, and rod shaped microcapsules of RLC-18 cells were produced for long-term culture to obtain desired morphologies of cell aggregates. |
| Databáze: | OpenAIRE |
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