Acute Effect of Sidestream Cigarette Smoke Extract on Vascular Endothelial Function
Autor: | Dionysios Adamopoulos, Jeanine Fontaine, A Ajose, Jean-François Argacha, Guy Berkenboom, D Fontaine, P. van de Borne |
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Rok vydání: | 2008 |
Předmět: |
Male
Nicotine medicine.medical_specialty Passive smoking Endothelium Vasodilator Agents Aorta Thoracic medicine.disease_cause chemistry.chemical_compound Superoxides Smoke Internal medicine Tobacco medicine Animals Rats Wistar Endothelial dysfunction Pharmacology Dose-Response Relationship Drug Chemistry Superoxide medicine.disease Acetylcholine Rats Vasodilation Oxidative Stress Dose–response relationship Endocrinology medicine.anatomical_structure Anesthesia Toxicity Tobacco Smoke Pollution Endothelium Vascular Cardiology and Cardiovascular Medicine medicine.drug |
Zdroj: | Journal of Cardiovascular Pharmacology. 52:262-267 |
ISSN: | 0160-2446 |
Popis: | Acute exposure to passive smoking adversely affects vascular function by promoting oxidative stress and endothelial dysfunction. However, it is not known whether tobacco sidestream (SS) smoke has a greater deleterious effect on the endothelium than non-tobacco SS smoke and whether these effects are related to nicotinic endothelial stimulation. To test these hypotheses, endothelial-dependent relaxation and superoxide anion production were assessed in isolated rat aortas incubated with tobacco SS smoke, non-tobacco SS smoke, or pure nicotine. Tobacco SS smoke decreased the maximal relaxation to acetylcholine (Ach) from 79 +/- 6% to 57 +/- 7.3% (% inhibition of phenylephrine-induced plateau, P < 0.001) and increased superoxide anion production from 31 +/- 9.7 to 116 +/- 24 count/10 sec/mg (P < 0.01, lucigenin-enhanced chemiluminescence technique). The non-tobacco SS smoke extract had no significant effect on the response to Ach but increased superoxide anion production in the aortic wall to 133 +/- 2 count/10 sec/mg (P < 0.001). Furthermore, concentration-response curves to Ach and superoxide production remained unaltered with nicotine (0.001, 0.01, or 0.1 mM). In conclusion, despite similar increases in vascular wall superoxide production with tobacco and non-tobacco SS smoke, only the tobacco SS smoke extracts affected endothelium-dependent vasorelaxation. Nicotine alone does not reproduce the effects seen with tobacco SS smoke, suggesting that the acute endothelial toxicity of passive smoking cannot simply be ascribed to a nicotine-dependent mechanism. |
Databáze: | OpenAIRE |
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