Analysis of Peripheral Blood Lymphocyte Subsets in Normal Saudi Males by Flow Cytometry
| Autor: | Khalid Al-Hussein, Kirtikant V. Sheth, Jennifer Lee, A. Al-Dalaan, Afrozul Haq, Sultan Al-Sedairy |
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| Rok vydání: | 1992 |
| Předmět: | |
| Zdroj: | Annals of Saudi Medicine. 12:540-543 |
| ISSN: | 0975-4466 0256-4947 |
| DOI: | 10.5144/0256-4947.1992.540 |
| Popis: | Lymphocyte immunophenotyping using flow cytometer has become an important tool for clinical patient management as well as for research and epidemiological studies. We examined the distribution of CD3 (all T cells), CD4 (T helper/inducer cells), CD8 (T suppressor/totoxic cells), CD16 (natural killer cells) and CD19 (B cells) in 150 healthy Saudi male blood donors using flows cytometry. The two-color labeled cells were analyzed by using the flow cytometer (FACScan, Becton-Dickinson, San Jose, California, USA) and the dual fluorescent subsets were discriminated by Simultest software. The distribution of T lymphocytes, B lymphocytes, and natural killer (NK) cells were similar to those reported in other populations as well as in normal Caucasian expatriate donors (all males) (n = 40) who were included in this study as controls. However, a significantly decreased CD4/CD8 ratio was observed in most Saudi blood donors. These lower ratios were due to decreased CD4 together with an increase in CD8 cells. Significant (P0.00001) difference in CD4/CD8 ratio in our study may be due to environmental factors such as ultraviolet radiation and stress (heat) as well as some genetic factors. |
| Databáze: | OpenAIRE |
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