Reduced neuropathy target esterase in pre‐eclampsia suppresses tube formation of HUVECs via dysregulation of phospholipid metabolism

Autor: Haibin Kuang, Yan Ling, Xin Shen, Yuezhen Li, Min Tang, Bei Yang, Hui Liu, Mo Li, Siying Lu
Rok vydání: 2020
Předmět:
Adult
0301 basic medicine
Physiology
Angiogenesis
Placenta
Clinical Biochemistry
Neovascularization
Physiologic
Neuropathy target esterase
Umbilical vein
Small hairpin RNA
Young Adult
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Pre-Eclampsia
Western blot
Cell Movement
Pregnancy
Human Umbilical Vein Endothelial Cells
medicine
Humans
Cells
Cultured
Phospholipids
Tube formation
biology
medicine.diagnostic_test
Lysophosphatidylcholines
Cell Biology
Glycerylphosphorylcholine
Molecular biology
030104 developmental biology
Lysophosphatidylcholine
medicine.anatomical_structure
chemistry
Phospholipases
Case-Control Studies
030220 oncology & carcinogenesis
embryonic structures
Phosphatidylcholines
cardiovascular system
biology.protein
Female
Carboxylic Ester Hydrolases
Signal Transduction
Zdroj: Journal of Cellular Physiology. 236:4435-4444
ISSN: 1097-4652
0021-9541
DOI: 10.1002/jcp.30160
Popis: Recently, studies have shown that neuropathy target esterase (NTE) is essential to placental and normal blood vessel development. However, whether it is involved in abnormal placenta angiogenesis of pre-eclampsia remains unknown. Thus, our aim was to observe the expression of NTE in pre-eclamptic placentas and its effects and mechanism of NTE on the migration and the tube formation of human umbilical vein endothelial cells (HUVECs). Immunohistochemical staining showed that the NTE protein was intensely located in blood vessels of the normal pregnant placenta. However, western blot revealed that the expression level of NTE protein was significantly reduced in pre-eclamptic placenta. The results indicated that overexpression of NTE significantly promoted the migration and the tube formation of HUVECs compared with those of the control and scramble short hairpin RNA (shRNA) group. Conversely, NTE shRNA obviously inhibited the migration and the tube formation of HUVECs. Additionally, chromatography assay evidenced that NTE overexpression significantly reduced the level of phosphatidylcholine (PC) of HUVECs, but NTE shRNA obviously increased the level of PC of HUVECs. Furthermore, exogenous PC and lysophosphatidylcholine (LPC) significantly inhibited the tube formation of HUVECs in a dose-dependent manner. Collectively, our results suggest that reduced NTE in placenta may contribute to abnormal placenta angiogenesis of pre-eclampsia via the dysregulation of PC and LPC metabolism.
Databáze: OpenAIRE
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