Determination of blood dexmedetomidine in dried blood spots by LC-MS/MS to screen therapeutic levels in paediatric patients

Autor: Luz María Torres-Espíndola, Raquel García-Álvarez, Liliana Rivera-Espinosa, Gustavo Lugo-Goytia, Josefina Gómez-Garduño, Radamés Alemón-Medina, Alejandra Toledo-López, María-Gabriela Pérez-Guillé, Juan Luis Chávez-Pacheco, Hugo Juárez-Olguín
Rok vydání: 2018
Předmět:
Physiology
Carboxylic Acids
Hematocrit
Tandem mass spectrometry
01 natural sciences
Pediatrics
chemistry.chemical_compound
0302 clinical medicine
030202 anesthesiology
Tandem Mass Spectrometry
Adrenergic alpha-2 Receptor Agonists
Medicine and Health Sciences
Hypnotics and Sedatives
Chromatography
High Pressure Liquid
Multidisciplinary
Spots
medicine.diagnostic_test
Chemistry
Organic Compounds
Pharmaceutics
Analgesics
Non-Narcotic
Reference Standards
Dried blood spot
Body Fluids
Blood
Child
Preschool
Physical Sciences
Medicine
Engineering and Technology
medicine.symptom
Anatomy
Dexmedetomidine
medicine.drug
Research Article
Quality Control
Drug Administration
Formic acid
Sedation
Science
Surgical and Invasive Medical Procedures
03 medical and health sciences
Drug Therapy
Industrial Engineering
Nitriles
medicine
Humans
Pharmacokinetics
Acetonitrile
Pharmacology
Chromatography
010401 analytical chemistry
Extraction (chemistry)
Organic Chemistry
Formic Acid
Infant
Newborn
Chemical Compounds
Infant
Biology and Life Sciences
0104 chemical sciences
Dried Blood Spot Testing
Acids
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 1, p e0210391 (2019)
ISSN: 1932-6203
Popis: Dexmedetomidine is an imidazole derivative, with high affinity for α2 adrenergic receptors, used for sedation, analgesia and adjuvant anaesthesia. In this study, an analytical method for the quantification of dexmedetomidine in dried blood spots was developed, validated and applied. The drug was extracted from dried blood spot by liquid extraction; the separation was carried out by ultra high-resolution liquid chromatography in reverse phase coupled to tandem mass spectrometry method. An X Select cyano 5 μm HSS column (2.1 X 150 mm, Waters) and a mobile phase composed of 0.1% formic acid: acetonitrile [50:50 v/v], were used. The test was linear over the concentration range of 50 to 2000 pg/mL. The coefficients of variation for the intra and interday trials were less than 15%. The drug was stable under the conditions tested. The method was successfully applied for the quantification of 6 patients, aged 0 to 2 years, with classification ASA I, who underwent ambulatory surgeries, receiving a dose of 1 μg/Kg dexmedetomidine IV. The drug concentrations in the different sampling times were in the range of 76 to 868 pg/mL.
Databáze: OpenAIRE
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