Determination of blood dexmedetomidine in dried blood spots by LC-MS/MS to screen therapeutic levels in paediatric patients
Autor: | Luz María Torres-Espíndola, Raquel García-Álvarez, Liliana Rivera-Espinosa, Gustavo Lugo-Goytia, Josefina Gómez-Garduño, Radamés Alemón-Medina, Alejandra Toledo-López, María-Gabriela Pérez-Guillé, Juan Luis Chávez-Pacheco, Hugo Juárez-Olguín |
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Rok vydání: | 2018 |
Předmět: |
Physiology
Carboxylic Acids Hematocrit Tandem mass spectrometry 01 natural sciences Pediatrics chemistry.chemical_compound 0302 clinical medicine 030202 anesthesiology Tandem Mass Spectrometry Adrenergic alpha-2 Receptor Agonists Medicine and Health Sciences Hypnotics and Sedatives Chromatography High Pressure Liquid Multidisciplinary Spots medicine.diagnostic_test Chemistry Organic Compounds Pharmaceutics Analgesics Non-Narcotic Reference Standards Dried blood spot Body Fluids Blood Child Preschool Physical Sciences Medicine Engineering and Technology medicine.symptom Anatomy Dexmedetomidine medicine.drug Research Article Quality Control Drug Administration Formic acid Sedation Science Surgical and Invasive Medical Procedures 03 medical and health sciences Drug Therapy Industrial Engineering Nitriles medicine Humans Pharmacokinetics Acetonitrile Pharmacology Chromatography 010401 analytical chemistry Extraction (chemistry) Organic Chemistry Formic Acid Infant Newborn Chemical Compounds Infant Biology and Life Sciences 0104 chemical sciences Dried Blood Spot Testing Acids |
Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 1, p e0210391 (2019) |
ISSN: | 1932-6203 |
Popis: | Dexmedetomidine is an imidazole derivative, with high affinity for α2 adrenergic receptors, used for sedation, analgesia and adjuvant anaesthesia. In this study, an analytical method for the quantification of dexmedetomidine in dried blood spots was developed, validated and applied. The drug was extracted from dried blood spot by liquid extraction; the separation was carried out by ultra high-resolution liquid chromatography in reverse phase coupled to tandem mass spectrometry method. An X Select cyano 5 μm HSS column (2.1 X 150 mm, Waters) and a mobile phase composed of 0.1% formic acid: acetonitrile [50:50 v/v], were used. The test was linear over the concentration range of 50 to 2000 pg/mL. The coefficients of variation for the intra and interday trials were less than 15%. The drug was stable under the conditions tested. The method was successfully applied for the quantification of 6 patients, aged 0 to 2 years, with classification ASA I, who underwent ambulatory surgeries, receiving a dose of 1 μg/Kg dexmedetomidine IV. The drug concentrations in the different sampling times were in the range of 76 to 868 pg/mL. |
Databáze: | OpenAIRE |
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