Iron-dependent reconfiguration of the proteome underlies the intracellular lifestyle of Brucella abortus
| Autor: | T. G. Alefantis, Vito G. DelVecchio, Mara S. Roset, Gabriel Briones |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Proteomics
0301 basic medicine Proteases Proteome Otras Ciencias Biológicas Iron 030106 microbiology lcsh:Medicine Brucella abortus Brucella Biology Protein aggregation Article Microbiology purl.org/becyt/ford/1 [https] Ciencias Biológicas 03 medical and health sciences Bacterial Proteins Stress Physiological Tandem Mass Spectrometry Animals proteoma lcsh:Science purl.org/becyt/ford/1.6 [https] Macrofagos Regulation of gene expression Multidisciplinary Macrophages Intracellular parasite lcsh:R Reproducibility of Results Gene Expression Regulation Bacterial intracelular biology.organism_classification Respiratory burst Host-Pathogen Interactions lcsh:Q Energy Metabolism CIENCIAS NATURALES Y EXACTAS Metabolic Networks and Pathways Intracellular Chromatography Liquid |
| Zdroj: | Scientific Reports CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Brucella ssp. is a facultative intracellular pathogen that causes brucellosis, a worldwide zoonosis that affects a wide range of mammals including humans. A critical step for the establishment of a successful Brucella infection is its ability to survive within macrophages. To further understand the mechanisms that Brucella utilizes to adapt to an intracellular lifestyle, a differential proteomic study was performed for the identification of intracellular modulated proteins. Our results demonstrated that at 48 hours post-infection Brucella adjusts its metabolism in order to survive intracellularly by modulating central carbon metabolism. Remarkably, low iron concentration is likely the dominant trigger for reprogramming the protein expression profile. Up-regulation of proteins dedicated to reduce the concentration of reactive oxygen species, protein chaperones that prevent misfolding of proteins, and proteases that degrade toxic protein aggregates, suggest that Brucella protects itself from damage likely due to oxidative burst. This proteomic analysis of B. abortus provides novel insights into the mechanisms utilized by Brucella to establish an intracellular persistent infection and will aid in the development of new control strategies and novel targets for antimicrobial therapy. Fil: Roset, Mara Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Alefantis, T. G.. Vital Probes; Estados Unidos Fil: Delvecchio, V. G.. Vital Probes; Estados Unidos Fil: Briones, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|