Modulation of the superoxide anion production and MMP-9 expression in PMA stimulated THP-1 cells by olive oil minor components: Tyrosol and hydroxytyrosol
| Autor: | Samia Dabbou, Francis Fouchier, Amel Nakbi, Sounira Mehri, N. Attia, Claude L. Léger, Mohamed Hammami, Serges Champion |
|---|---|
| Přispěvatelé: | Université de Monastir (Université de Monastir), Nutriments Lipidiques et Prévention des Maladies Métaboliques, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Montpellier 1 (UM1), Ministere de l'Enseignement Superieur et de la Recherche Scientifique Tunisia [UR03/ES-08] |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Antioxidant
medicine.medical_treatment [SDV]Life Sciences [q-bio] LOW-DENSITY-LIPOPROTEIN INHIBITION KAPPA-B ACTIVATION chemistry.chemical_compound POLYPHENOLS ANTIOXIDANT ACTIVITIES medicine Tyrosol NADPH OXIDASE Zymography Hydroxytyrosol Lucigenin OXIDATIVE STRESS chemistry.chemical_classification Anion superoxide Reactive oxygen species Superoxide Monocyte PHENOLIC-COMPOUNDS Molecular biology medicine.anatomical_structure chemistry Biochemistry CACO-2 CELLS Monocyte THP-1 cells MMP-9 Food Science JUICE |
| Zdroj: | Food Research International Food Research International, Elsevier, 2011, 44 (2), pp.575-581. ⟨10.1016/j.foodres.2010.12.010⟩ |
| ISSN: | 0963-9969 |
| DOI: | 10.1016/j.foodres.2010.12.010⟩ |
| Popis: | Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; Extra virgin olive oil has been associated with a reduced incidence of risk factors for coronary heart disease also owing to the presence of antioxidant biophenols. Reactive oxygen species (ROS) and matrix metalloproteinase-9 (MMP-9) have been implicated in numerous somatic illnesses, including cardiovascular disorders and cancer. The aim of this work was to study the capacity of virgin olive oil tyrosol (T) and hydroxytyrosol (HT) at impairing superoxide production and MMP-9 expressions in monocyte cells (THP-1) conveniently differentiated into adherent macrophages, taken as a model of human macrophages implicated in atheroma. O(2)(center dot-) production was evaluated in THP-1 cells by using lucigenin as a specific chemiluminescent probe. Cells, after differentiation for 72 h, were preincubated in the presence of HT and Tat increasing concentrations for 4, 15 and 24 h, and then, monocyte-like cells were stimulated by phorbol myristate acetate (PMA) and the O(2)(center dot-)-dependent luminescence was immediately recorded at 37 degrees C by means of a Luminometer. Enzymatic activity of MMP-9 derived from a medium of cells preincubated, or not, with T or HT was tested by zymography. As compared to the cells without treatment, cells preincubated with HT, showed a decrease of O(2)(center dot-) production (50%) at 1 mu M for 15 h of preincubation time. Tyrosol fully prevented ROS overproduction at 15 h and, like HT displayed a high degree of protection but at higher concentrations and later time points (24 h). Gelatin zymograms revealed a reduction of the expression of MMP-9 in conditioned medium derived from T and HT-treated cells. These findings give further evidence in favour of olive oil consumption to counteract cardiovascular diseases. (C) 2010 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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