Some biochemical properties of an acyclic oligonucleotide analogue. A plausible ancestor of the DNA?
| Autor: | Gérard Spach, Attila Szemzo, Yves Merle, Liliane Merle, Janos Sagi |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
biology DNA polymerase Oligonucleotide Phosphoric Diester Hydrolases Hydrolysis RNA Nucleic Acid Precursors General Medicine DNA-Directed DNA Polymerase Polymerase Chain Reaction Substrate Specificity Deoxyribonucleoside chemistry.chemical_compound Enzyme Biochemistry chemistry Space and Planetary Science Nucleic acid biology.protein Thymine Nucleotides Primer (molecular biology) Ecology Evolution Behavior and Systematics DNA |
| Zdroj: | Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life. 23(2) |
| ISSN: | 0169-6149 |
| Popis: | As acyclic oligonucleotides have been suggested as a primitive model of DNA or RNA in prebiotic times, we compared some biochemical properties of these analogues to that of natural ones. Firstly, an acyclic analogue of deoxyribonucleoside triphosphates was tested as a potential substrate of enzymes intervening in nucleic acids synthesis. GlyTTP, a dTTP analogue with a missing 2'-methylene group is not accepted as a substrate by either DNA polymerase or deoxynucleotidyl terminal transferase (TdT). Secondly, the modified dodecathymidylate (GlyT)12, the racemic acyclic sugar analogue of (dT)12, proved to be an efficient primer for DNA polymerase and TdT, though the associative properties of (GlyT)12 are very weak as shown by UV spectroscopy in phosphate buffer without magnesium chloride. But (GlyT)12 has the advantage to be 500-times more stable against hydrolysis by snake venom phosphodiesterase than the corresponding oligothymidylate. |
| Databáze: | OpenAIRE |
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