Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells
| Autor: | Kalpana Joshi, Ramesh R. Bhonde, Avinash Sanap |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Medicine (General) medicine.medical_treatment Glucose uptake 030204 cardiovascular system & hematology 03 medical and health sciences 0302 clinical medicine Insulin resistance R5-920 Internal medicine Type 2 diabetes mellitus medicine Interferon gamma Receptor Dexamethasone Inflammation biology business.industry Insulin medicine.disease Endocrinology Oxidative stress 030220 oncology & carcinogenesis biology.protein Mesenchymal stem cells Original Article Tumor necrosis factor alpha business GLUT4 medicine.drug |
| Zdroj: | Chronic Diseases and Translational Medicine, Vol 7, Iss 1, Pp 47-56 (2021) Chronic Diseases and Translational Medicine |
| Popis: | Background: Pharmacological factors used to induce insulin resistance (IR) in in vitro models may not mimic the full in vivo features of type 2 diabetes mellitus (T2DM). This study aimed to examine the ability of diabetic serum (DS) to induce IR and investigate whether adipose-derived mesenchymal stem cell conditioned medium (ADMSC-CM) reverses DS-induced IR. Methods: DS was obtained from newly diagnosed T2DM patients. IR was induced in differentiated 3T3-L1 cells by employing dexamethasone, tumor necrosis factor alpha (TNF-α), palmitate and DS. Glucose uptake (2-[N-[7-nitrobenz-2-oxa-1,3-diazol-4-yl] amino]-2-deoxyglucose(2-NBDG) uptake assay), intracellular levels of reactive oxygen species (ROS), and superoxide radicals (O2−) (fluorescence microscopy and fluorometry) were analyzed in control and experimental samples. mRNA expression of key genes involved in glucose transport and inflammation were analyzed by using reverse transcription polymerase chain reaction (RT-PCR). Pro-inflammatory cytokines and phospho-insulin receptor substrate (IRS) (Ser-307) protein expression were analyzed by fluorescence activated cell sorter analysis. Statistical significance was determined by using one-way ANOVA followed by Tukey's multiple comparison tests. Results: ADMSC-CM significantly increased the DS-mediated decrease in 2-NBDG uptake (11.01 ± 0.50 vs. 7.20 ± 0.30, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|