Analysis of BCR/ABL transcripts in chronic myeloid patients by nested PCR using an immunoassay-like detection format
| Autor: | Véronique Barbu, Béatrice Alpha-Bazin, Camille Chypre, Laurence Morand-Joubert, Dominique Pepin, Gilbert Bereziat |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Myeloid
Transcription Genetic Molecular Sequence Data Pcr cloning Fusion Proteins bcr-abl Biology Chronic myeloid leukaemia Polymerase Chain Reaction Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases medicine Humans RNA Messenger Molecular Biology Immunoassay ABL Routine screening Base Sequence medicine.diagnostic_test Chimera breakpoint cluster region DNA Neoplasm Cell Biology Molecular biology Blotting Southern medicine.anatomical_structure Nested polymerase chain reaction |
| Zdroj: | Molecular and Cellular Probes. 8:251-256 |
| ISSN: | 0890-8508 |
| DOI: | 10.1006/mcpr.1994.1036 |
| Popis: | We describe an 'immunoassay-like' detection format, based of the AMPLICIS technique for the routine detection of BCR/ABL transcripts in leukaemic patients. The AMPLICIS technique is characterized as a nested PCR couple to solid phase isotopic or non-isotopic detection of PCR products. Comparison between this assay and a conventional PCR technique using Southern-blot analysis provided a good correlation between the two procedures. The assay, easier and faster than the conventional one, gives access to automation and therefore appears well suited for the routine screening of BCR/ABL chimaeric mRNAs. |
| Databáze: | OpenAIRE |
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