Involvement of phosphoinositide turnover in tracheary element differentiation in Zinnia elegans L. cells
Autor: | Gary G. Coté, Richard C. Crain, Xianchao G. Zhang |
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Rok vydání: | 2002 |
Předmět: |
Programmed cell death
Cellular differentiation Inositol 1 4 5-Trisphosphate Plant Science Asteraceae Phosphatidylinositols chemistry.chemical_compound Tracheary element differentiation Genetics Inositol Virulence Factors Bordetella Estrenes Inositol phosphate chemistry.chemical_classification Phospholipase C biology Transdifferentiation Zinnia elegans Cell Differentiation biology.organism_classification Pyrrolidinones Pertussis Toxin chemistry Biochemistry Type C Phospholipases Calcium Plant Structures |
Zdroj: | Planta. 215:312-318 |
ISSN: | 1432-2048 0032-0935 |
Popis: | Mesophyll cells of Zinnia elegans L., cultured in the presence of phytohormones, will transdifferentiate and undergo programmed cell death to become tracheary elements, thick-walled cells of the xylem. This system is a model system for study of plant cell development and differentiation. We report that a high concentration of extracellular Ca(2+) is necessary during the first 6 h of culturing for tracheary elements to form. Extracellular Ca(2+) is still required at later times, but at a much lower concentration. When cells transdifferentiate in adequate Ca(2+), microsomal phospholipase C activity increases and levels of inositol 1,4,5-trisphosphate rise at about hour 4 of culturing. The production of inositol 1,4,5-trisphosphate appears to be important for tracheary element formation, since inhibitors of phospholipase C inhibit both inositol 1,4,5-trisphosphate production and tracheary element formation. Pertussis toxin, an inhibitor of GTP-binding proteins, inhibits transdifferentiation and eliminates inositol 1,4,5-trisphosphate production. Tracheary element formation was not completely abolished by inhibitors that eliminated inositol 1,4,5-trisphosphate production, suggesting the involvement of other pathways in regulating transdifferentiation. |
Databáze: | OpenAIRE |
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