Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
| Autor: | Joseph M. Kinsella, Jiang Tao, Antonio De León-Rodríguez, Jose G. Munguia-Lopez, Joel Grant, Salvador Flores-Torres, Sanahan Vijayakumar |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Stromal cell food.ingredient Alginates Science 02 engineering and technology Gelatin Article law.invention 03 medical and health sciences food law Neoplasms Spheroids Cellular Tumor Cells Cultured Tumor Microenvironment medicine Humans Fibroblast Triple-negative breast cancer 3D bioprinting Multidisciplinary Tissue Scaffolds Chemistry Bioprinting Spheroid Hydrogels 021001 nanoscience & nanotechnology Coculture Techniques In vitro Cell biology 030104 developmental biology medicine.anatomical_structure Immunology Self-healing hydrogels Medicine 0210 nano-technology |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Human tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a method to embed MDA-MB-231 triple negative breast cancer cells, and IMR-90 fibroblast cells, within a cross-linked alginate/gelatin matrix at specific initial locations relative to each other. After 7 days of co-culture the MDA-MB-231 cells begin to form multicellular tumour spheroids (MCTS) that increase in size and frequency over time. After ~15 days the IMR-90 stromal fibroblast cells migrate through a non-cellularized region of the hydrogel matrix and infiltrate the MDA-MB-231 spheroids creating mixed MDA-MB-231/IMR-90 MCTS. This study provides a proof-of-concept that biomimetic in vitro tissue co-culture models bioprinted with both breast cancer cells and fibroblasts will result in MCTS that can be maintained for durations of several weeks. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|