Analysis of disease-associated amino acid epitopes on HLA class II molecules in atopic dermatitis
| Autor: | Takeo Juji, Hidehisa Saeki, Takafumi Etoh, Mitsuko Miyamoto, Masami Yanagisawa, Shoji Kuwata, Yoichi Shibata, Katsushi Tokunaga, Hidemi Nakagawa |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
musculoskeletal diseases
Adult Male Allergy Adolescent Genotype Immunology Molecular Sequence Data Human leukocyte antigen Immunoglobulin E Polymerase Chain Reaction Epitope Dermatitis Atopic Epitopes immune system diseases HLA-DQ Antigens medicine Immunology and Allergy HLA-DQ beta-Chains Humans Amino Acid Sequence Allele Amino Acids Child Alleles chemistry.chemical_classification biology Base Sequence Haplotype Atopic dermatitis HLA-DR Antigens Middle Aged medicine.disease Molecular biology Amino acid chemistry biology.protein Female Polymorphism Restriction Fragment Length HLA-DRB1 Chains |
| Zdroj: | The Journal of allergy and clinical immunology. 96(6 Pt 2) |
| ISSN: | 0091-6749 |
| Popis: | We investigated the association between HLA class II alleles and severe atopic dermatitis with high serum IgE levels (greater than 8000 U/ml). The frequencies of HLA-DRB1*1302 and DQB1*0604 were increased, whereas the frequency of HLA-DQB1*0302 was decreased. A strong haplotype, HLA-DRB1*1302-DQB1*0604, has been reported in the Japanese population, and this haplotype is conserved in patients with AD. Further analysis of the amino acid epitopes on the HLA-DR beta 1 and DQ beta 1 domains revealed that DR beta 1 71Glu (RR = 5.71, p0.05) and DQ beta 1 30His (RR = 3.25 p0.01), and 57Val (RR = 3.13, p0.05) were increased in frequency. DR beta 1 71Glu was exclusively unique to DRB1*1302. DQ beta 1 30His was shared by the following alleles: DQ beta 1*0501, *0502, *0503, and *0604, which were all increased in patients with AD. DQ beta 1 57Val was shared by DQB1*0501 and *0604. A well-known haplotype, HLA-DRB1*1302-DQB1*0604, contains DR beta 1 71Glu and DQ beta 1 30His and 57Val. Therefore HLA-DR beta 1 71Glu and/or DQ beta 1 30His/57Val are considered to play the most important role in the development of atopic dermatitis. |
| Databáze: | OpenAIRE |
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