Study of acetylated histone h3k9 – an active chromatin mark – in chromosomes from adult and fetal human lymphocytes
| Autor: | Yuliia G. Lezhnina, Olga A. Efimova, Olga G. Chiryaeva, Mikhail I. Krapivin, Vladislav S Baranov, Anna A. Pendina, Anastasiia V. Petrovskaia-Kaminskaia, Tatiana V. Kuznetzova, Vera S. Dudkina, Alla S. Koltsova, Lyubov I. Petrova, Andrei V. Tikhonov, Olga E. Talantova |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
chromosomes
lcsh:QH426-470 Health Toxicology and Mutagenesis Biology Biochemistry Andrology 03 medical and health sciences 0302 clinical medicine Genetics Epigenetics Metaphase Genetics (clinical) Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Fetus prenatal diagnosis Ecology epigenetics histone acetylation Chromosome Agricultural and Biological Sciences (miscellaneous) Chromatin lcsh:Genetics human development Acetylation 030220 oncology & carcinogenesis Cord blood Human genome Biotechnology chromatin disorders |
| Zdroj: | Экологическая генетика, Vol 17, Iss 3, Pp 111-117 (2019) |
| ISSN: | 2411-9202 1811-0932 |
| Popis: | Background: Incorrect epigenetic modifications of the human genome may result in epigenetic disorders, thus, highlighting the necessity of studying chromosome epigenetic patterns in human development. Aim of the study: A comparative analysis of acetylated histone H3K9 (AcH3K9) patterns in human metaphase chromosomes from the lymphocytes of adults and fetuses. Materials and methods: The immunocytochemical detection of AcH3K9 in the metaphase chromosomes from PHA-stimulated peripheral lymphocytes of 13 adults and cord blood lymphocytes of 10 fetuses at 20-22 weeks of gestation. Results: Both in the chromosomes of the adults and the fetuses, AcH3K9 accumulated in the R- and T-, but not G-bands and avoided the regions of pericentromeric heterochromatin of the chromosomes 1, 9 and 16. When comparing the adult and the fetal chromosomes, different levels of AcH3K9 were revealed in a few bands: 2q31, 5p13, 5p15 and 16p13 had higher level of Н3К9 acetylation in adults, in contrast to 9q13 which was hyperacetylated in fetuses. Conclusion: The АсН3К9 distribution in metaphase chromosomes is band-specific and is similar between the adults and the fetuses, excluding a few bands with different acetylation levels. |
| Databáze: | OpenAIRE |
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