Natural selection between day 3 and day 5/6 PGD embryos in couples with reciprocal or Robertsonian translocations
| Autor: | Claire E. Beyer, E. Willats |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Pregnancy Rate Reproductive medicine Robertsonian translocation Embryonic Development Chromosomal translocation Fertilization in Vitro Biology Preimplantation genetic diagnosis medicine.disease_cause Translocation Genetic Chromosome segregation 03 medical and health sciences 0302 clinical medicine Pregnancy Chromosome Segregation Genetics medicine Humans Selection Genetic Genetics (clinical) In Situ Hybridization Fluorescence Preimplantation Diagnosis 030219 obstetrics & reproductive medicine Natural selection Obstetrics and Gynecology Embryo General Medicine Embryo Transfer Human genetics Embryo Biology 030104 developmental biology Reproductive Medicine Female Developmental Biology |
| Zdroj: | Journal of assisted reproduction and genetics. 34(11) |
| ISSN: | 1573-7330 |
| Popis: | For translocation carriers, preimplantation genetic diagnosis (PGD) provides the opportunity to distinguish between normal/balanced and unbalanced embryos prior to implantation and, as such, increases the likelihood of a successful ongoing pregnancy. The data presented here compares autosomal reciprocal and Robertsonian translocation segregation patterns in day 3 versus day 5/6 IVF-PGD embryos to determine if there is a difference in the chromosome segregation patterns observed at these developmental time points.A retrospective analysis on PGD translocation carriers at Monash IVF was performed. Segregation patterns were compared between day 3 and day 5/6 embryos to ascertain whether selection against malsegregants exists.For reciprocal translocations, 1649 day 3 embryos (139 translocations) from 144 couples and 128 day 5/6 embryos (59 translocations) from 60 couples were analysed. Day 3 segregation analysis showed that 22.3% of embryos were normal/balanced (consistent with 2:2 alternate segregation) and 77.7% were unbalanced (malsegregation). Day 5/6 segregation analysis showed that 53.1% of embryos were normal/balanced and 46.9% were unbalanced. For Robertsonian translocations, 847 day 3 embryos (8 translocations) from 54 couples and 193 day 5/6 embryos (6 translocations) from 31 couples were analysed. Day 3 segregation analysis showed that 38.7% of embryos were normal/balanced (consistent with 2:1 alternate segregation) and 61.3% were unbalanced. Day 5/6 segregation analysis showed that 74.1% of embryos were normal/balanced and 25.9% were unbalanced.This data demonstrates an increase in the proportion of genetically normal/balanced embryos at day 5/6 of development. This suggests a strong natural selection process between day 3 and day 5/6 in favour of normal/balanced embryos. These findings support performing PGD testing on day 5/6 of embryo development. |
| Databáze: | OpenAIRE |
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