Rapid repair of DNA double strand breaks in Arabidopsis thaliana is dependent on proteins involved in chromosome structure maintenance

Autor: Christopher E. West, Jaroslav Kozak, José A. da Costa-Nunes, Karel J. Angelis, Charles I. White
Přispěvatelé: Institute of Experimental Botany of the Czech Academy of Sciences (IEB / CAS), Czech Academy of Sciences [Prague] (CAS), CPS, University of Leeds, Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), ISA (ISA), Universidade de Lisboa (ULISBOA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Universidade de Lisboa = University of Lisbon (ULISBOA), White, Charles, Instituto Superior de Agronomia [Lisboa] (ISA)
Rok vydání: 2008
Předmět:
0106 biological sciences
Time Factors
DNA Repair
Chromosomal Proteins
Non-Histone
genetic processes
MESH: DNA Breaks
Double-Stranded
Arabidopsis
[SDV.GEN] Life Sciences [q-bio]/Genetics
01 natural sciences
Biochemistry
MESH: Dose-Response Relationship
Drug
chemistry.chemical_compound
MESH: Arabidopsis
DNA Breaks
Double-Stranded
MESH: Chromosomes
Plant
MESH: DNA Repair
Recombination
Genetic
0303 health sciences
Antibiotics
Antineoplastic
DNA repair protein XRCC4
MESH: Bleomycin
Nuclear DNA
Cell biology
MESH: Recombination
Genetic
Comet Assay
biological phenomena
cell phenomena
and immunity
DNA
Plant
DNA damage
DNA repair
MESH: Comet Assay
MESH: Arabidopsis Proteins
DNA Fragmentation
Biology
Chromosomes
Plant
03 medical and health sciences
Bleomycin
MESH: Chromosomal Proteins
Non-Histone
MESH: DNA Fragmentation
MESH: Antibiotics
Antineoplastic
MESH: DNA
Plant
Molecular Biology
030304 developmental biology
[SDV.GEN]Life Sciences [q-bio]/Genetics
Dose-Response Relationship
Drug
Arabidopsis Proteins
SMC protein
fungi
MESH: Time Factors
Cell Biology
biology.organism_classification
Molecular biology
Comet assay
enzymes and coenzymes (carbohydrates)
chemistry
health occupations
DNA
010606 plant biology & botany
Zdroj: DNA Repair
DNA Repair, Elsevier, 2009, 8 (3), pp.413-9. ⟨10.1016/j.dnarep.2008.11.012⟩
DNA Repair, 2009, 8 (3), pp.413-9. ⟨10.1016/j.dnarep.2008.11.012⟩
ISSN: 1568-7864
Popis: International audience; DNA double strand breaks (DSBs) are one of the most cytotoxic forms of DNA damage and must be repaired by recombination, predominantly via non-homologous joining of DNA ends (NHEJ) in higher eukaryotes. However, analysis of DSB repair kinetics of plant NHEJ mutants atlig4-4 and atku80 with the neutral comet assay shows that alternative DSB repair pathways are active. Surprisingly, these kinetic measurements show that DSB repair was faster in the NHEJ mutant lines than in wild-type Arabidopsis. Here we provide the first characterization of this KU-independent, rapid DSB repair pathway operating in Arabidopsis. The alternate pathway that rapidly removes the majority of DSBs present in nuclear DNA depends upon structural maintenance of chromosomes (SMC) complex proteins, namely MIM/AtRAD18 and AtRAD21.1. An absolute requirement for SMC proteins and kleisin for rapid repair of DSBs in Arabidopsis opens new insight into the mechanism of DSB removal in plants.
Databáze: OpenAIRE
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