Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways
| Autor: | Gui-lian Fu, Hong Gao, Xiao-Hong Han, Shi-He Shao, Xian-qian Li |
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| Rok vydání: | 2010 |
| Předmět: |
genetic structures
Blotting Western Mitochondrial pathway Apoptosis Membrane Potentials Flow cytometry chemistry.chemical_compound Cell Line Tumor medicine Humans Pharmacology (medical) bcl-2-Associated X Protein Norcantharidin medicine.diagnostic_test Caspase 3 Liver Neoplasms Apoptosis Inducing Factor Cytochromes c General Medicine Bridged Bicyclo Compounds Heterocyclic Flow Cytometry medicine.disease digestive system diseases Mitochondria Cell biology Blot Complementary and alternative medicine chemistry Cell culture Liver cancer Hepatoma cell |
| Zdroj: | Chinese Journal of Integrative Medicine. 16:448-452 |
| ISSN: | 1993-0402 1672-0415 |
| DOI: | 10.1007/s11655-010-0538-5 |
| Popis: | To investigate the mechanism of norcantharidin (NCTD)-induced SMMC-7721 hepatoma cell apoptosis.SMMC-7721 cell growth inhibition was measured by the MTT method. Apoptosis was detected by Annexin V/propidium iodide staining. The mitochondrial membrane potential was measured by flow cytometry. Western blot analysis was used to evaluate the level of cytochrome c, caspase-3, AIF, Bcl-2 and Bax expression.NCTD inhibited SMMC-7721 cell growth in a time- and dose-dependent manner. The cells treated with NCTD showed the loss of mitochondrial membrane potential. The activities of caspase-3, cytochrome c, AIF, and Bax were up-regulated after NCTD treatment at different doses. The expression of Bcl-2 was decreased after treatment with NCTD.NCTD could induce SMMC-7721 cell apoptosis. The activation of the mitochondrial pathway was involved in the process of NCTD-induced SMMC-7721 cell apoptosis. |
| Databáze: | OpenAIRE |
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