Establishment of sperm associated antigen 6 gene knockout mouse model and its mechanism of deafness
Autor: | Qinshuang Zhou, Jun Lv, Lanlan Jin, Zonghui Kang, Yideng Huang, He Li |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine 01 natural sciences Article Hearing impairment 03 medical and health sciences Western blot medicine Pgrn genes Prestin Gene lcsh:QH301-705.5 Cochlea Gene knockout Otitis media Messenger RNA biology medicine.diagnostic_test Spag6 genes Molecular biology 030104 developmental biology Real-time polymerase chain reaction lcsh:Biology (General) Knockout mouse biology.protein sense organs Prestin genes General Agricultural and Biological Sciences 010606 plant biology & botany Knockout mice |
Zdroj: | Saudi Journal of Biological Sciences, Vol 27, Iss 5, Pp 1289-1295 (2020) Saudi Journal of Biological Sciences |
Popis: | To investigate the effects of knocking out the Sperm associated antigen6 (Spag6) gene on the auditory system of mice, the heterozygous type Spag6 knockout mouse model built in the previous period was used for mating and breeding, and homozygous type Spag6 gene knockout mouse (Spag−/−), heterozygous type Spag6 gene knockout mouse (Spag+/−) and wild type mouse (Spag+/+) were obtained. PCR technology was used to verify mouse models with different genotypes. After verification, the hearing threshold responses of Spag+/+ and Spag−/− genotype mice were detected. The localization of Spag6 gene in the basal membrane of the cochlea of the inner ear was detected by immunofluorescence staining. The changes of middle ear tissues were observed by H.E. staining sections. The relative expression of Prestin gene and Pgrn gene in different age mice was detected by fluorescence quantitative PCR. The relative expression of Prestin gene was detected by western blot. The results showed that Spag−/− mice had hearing impairment compared with Spag+/+ mice. And Spag6 protein is distributed in different genotypes of mouse hair cells; Spag−/− mice showed otitis media. The expression of Prestin mRNA and protein in Spag−/− mice was significantly higher than that in Spag+/+ mice (P |
Databáze: | OpenAIRE |
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