Alginate enhances Toll-like receptor 4-mediated phagocytosis by murine RAW264.7 macrophages
| Autor: | Decheng Bi, Qiuxian Lai, Jun Lu, Qingguo Han, Zhishu Tang, Xu Xu, Nan Cai, Hong Xu, Zedong Jiang, Weiyang Bao, Yanwen Peng, Rui Zhou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Staphylococcus aureus Alginates MAP Kinase Signaling System Phagocytosis Metal Nanoparticles Biology p38 Mitogen-Activated Protein Kinases Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glucuronic Acid Structural Biology Animals Macrophage Protein kinase A Molecular Biology Protein kinase B Toll-like receptor Hexuronic Acids Macrophages NF-kappa B NF-κB General Medicine Molecular biology Toll-Like Receptor 4 RAW 264.7 Cells 030104 developmental biology chemistry 030220 oncology & carcinogenesis TLR4 Gold Proto-Oncogene Proteins c-akt |
| Zdroj: | International Journal of Biological Macromolecules. 105:1446-1454 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2017.07.129 |
| Popis: | Alginate is a naturally acidic polysaccharide consisting alternately of β-d-mannuronic acid and α-l-guluronic acid with 1, 4-glycosidic linkages and is derived from brown seaweeds. Herein, the effect of alginate on the promotion of macrophage phagocytosis and the corresponding molecular mechanisms were investigated in murine RAW264.7 cells. Alginate could enhance the intracellular phagocytosis of gold nanoparticles (AuNPs), fluorescent microspheres and immunoglobulin G (IgG)-opsonized Staphylococcus aureus (S. aureus). Moreover, alginate increased Toll-like receptor 4 (TLR4) expression and activated the Akt/nuclear factor-κB (NF-κB) and p38 mitogen-activated protein kinase (MAPK) signalling pathways. Alginate-promoted phagocytosis was suppressed by the addition of inhibitors of TLR4, NF-κB and p38 MAPK and by TLR4 gene knockdown, indicating the involvement of these key components. This work is the first to propose that alginate promotes phagocytosis via upregulating TLR4 expression and stimulating the Akt/NF-κB and p38 MAPK signalling pathways, which may contribute to the capacity of alginate to activate macrophages. |
| Databáze: | OpenAIRE |
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