In Vivo Biocompatibility and Analytical Performance of Intravascular Amperometric Oxygen Sensors Prepared with Improved Nitric Oxide-Releasing Silicone Rubber Coating
| Autor: | Megan C. Frost, Martin A. Maraschio, Steven M. Rudich, Mark E. Meyerhoff, Huiping Zhang |
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| Rok vydání: | 2002 |
| Předmět: |
Biocompatibility
Swine engineering.material Nitric Oxide Electrochemistry Silicone rubber Analytical Chemistry chemistry.chemical_compound Catheters Indwelling Coated Materials Biocompatible Coating In vivo Animals Humans Nitric Oxide Donors chemistry.chemical_classification Chemistry technology industry and agriculture Polymer Amperometry Femoral Artery Oxygen Carotid Arteries Silicone Elastomers engineering Blood Gas Analysis Oxygen sensor Biomedical engineering |
| Zdroj: | Analytical Chemistry. 74:5942-5947 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | The in vivo biocompatibility and analytical performance of amperometric oxygen-sensing catheters prepared with a new type of nitric oxide (NO)-releasing silicone rubber polymer (DACA/N2O2 SR) is reported. The NO-release silicone rubber coating contains diazeniumdiolated secondary amine sites covalently anchored to a dimethylsiloxane matrix. Narrow diameter (0.9 mm, o.d.) silicone rubber tubing coated with this polymer can be employed to construct functional oxygen-sensing catheters that release NO continuously at levels1 x 10(-10) mol/cm2-min for more than 20 h. In vivo evaluation of such sensors within the carotid and femoral arteries of swine over a 16-h time period demonstrates that sensors prepared with the new NO-release coating exhibit no significant platelet adhesion or thrombus formation, but control sensors (non-NO release) implanted within the same animals do show a high propensity for cell adhesion and bulk clot formation. Furthermore, the in vivo analytical data provided by sensors fabricated with NO-release coatings (N = 9) are shown to be statistically equivalent to PO2 levels measured in vitro on discrete samples of blood. Control sensors (N = 9) placed within the same animals yield average PO2 values that are statistically different (por = 0.05) (lower) from both the levels measured on discrete samples and those provided by the NO-release sensors over a 16-h in vivo monitoring period. |
| Databáze: | OpenAIRE |
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