3D second harmonic generation imaging tomography by multi-view excitation
| Autor: | Benjamin L. Cox, Kirby R. Campbell, Kevin W. Eliceiri, Emily Shelton, Paul J. Campagnola, Robert Swader, Bruce Wen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
animal structures Materials science business.industry Resolution (electron density) Second-harmonic imaging microscopy Second-harmonic generation Image processing 01 natural sciences Article Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials 010309 optics 03 medical and health sciences Dipole 030104 developmental biology Optics Light sheet fluorescence microscopy 0103 physical sciences Microscopy Tomography business |
| Zdroj: | Optica. 4:1171 |
| ISSN: | 2334-2536 |
| DOI: | 10.1364/optica.4.001171 |
| Popis: | Biological tissues have complex 3D collagen fiber architecture that cannot be fully visualized by conventional second harmonic generation (SHG) microscopy due to electric dipole considerations. We have developed a multi-view SHG imaging platform that successfully visualizes all orientations of collagen fibers. This is achieved by rotating tissues relative to the excitation laser plane of incidence, where the complete fibrillar structure is then visualized following registration and reconstruction. We evaluated high frequency and Gaussian weighted fusion reconstruction algorithms, and found the former approach performs better in terms of the resulting resolution. The new approach is a first step toward SHG tomography. |
| Databáze: | OpenAIRE |
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