Recognition of a B-cell Epitope of the VapA Protein of Rhodococcus equi in Newborn and Experimentally Infected Foals
| Autor: | Michael W. Heuzenroeder, Mary D. Barton, Tongted Phumoonna |
|---|---|
| Přispěvatelé: | Barton, Mary Darvall, Heuzenroeder, Michael, Phumoonna,Tongted |
| Rok vydání: | 2005 |
| Předmět: |
Veterinary Medicine
animal diseases Immunoglobulins Infectious Agents Virulence Enzyme-Linked Immunosorbent Assay Epitope Plasmid Bacterial Proteins Predictive Value of Tests Rhodococcus equi parasitic diseases medicine Animals Horses Seroconversion B cell Veterinary Microbiology (excl Virology) biology General Medicine medicine.disease biology.organism_classification Virology Pneumonia medicine.anatomical_structure Animals Newborn biology.protein Epitopes B-Lymphocyte Horse Diseases Antibody Actinomycetales Infections Epitope Mapping |
| Zdroj: | Journal of Veterinary Medicine Series B. 52:291-295 |
| ISSN: | 1439-0450 0931-1793 |
| DOI: | 10.1111/j.1439-0450.2005.00858.x |
| Popis: | The aim of this study was to evaluate the usefulness of the previously identified B-cell epitope TSLNLQKDEPNGRASDTAGQ of the VapA protein of Rhodococcus equi and its association with R. equi pneumonia. A modified peptide designated PN11-14 corresponding to the epitope was recognized by all sera from experimentally infected foals with virulent R. equi ATCC103+ containing the virulence plasmid but not by its plasmid-cured derivative ATCC103- strain. Marked levels of VapA-specific immunoglobulin (Ig)G were detected in all sera from the ATCC103+ infected foals at 2 weeks after the infection. One control animal had high titres as determined by the peptide enzyme-linked immunosorbent assay (ELISA), indicating the ELISA may not absolutely differentiate between foals with R. equi pneumonia and healthy exposed foals in farms where the prevalence of disease is high. However, numbers of animals used were small. Further evaluation of the peptide ELISA with field samples is necessary to determine whether the assay is diagnostically useful. This study showed that levels of passive transfer of maternal IgG antibodies to the epitope in newborn foals could be measured. Interestingly, the maternally derived antibodies were found to significantly (P |
| Databáze: | OpenAIRE |
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