Nitrite is produced by elicited but not by circulating neutrophils
| Autor: | Christopher G. Sobey, R. G. Giarracca, Trudi Harris, Alastair G. Stewart, Y. Lim, Gregory J. Dusting |
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| Rok vydání: | 1993 |
| Předmět: |
biology
Glycogen business.industry Immunology Inflammation Cell Biology Molecular biology Nitric oxide Nitric oxide synthase chemistry.chemical_compound medicine.anatomical_structure chemistry Peritoneum lcsh:Pathology medicine biology.protein Tumor necrosis factor alpha medicine.symptom Nitrite business Phenylephrine Research Article lcsh:RB1-214 medicine.drug |
| Zdroj: | Mediators of Inflammation, Vol 2, Iss 5, Pp 349-356 (1993) Mediators of Inflammation |
| ISSN: | 1466-1861 0962-9351 |
| DOI: | 10.1155/s0962935193000481 |
| Popis: | The generation of nitrite (NO2−) was used as an index of the production of nitric oxide by human and rat polymorphonuclear leukocytes (PMN) and rat peritoneal macrophages. Human peripheral blood PMN did not produce significant levels ofNO2−. Attempts to induceNO2−generation in human PMN by incubation with GM–CSF (1 nM), TNFα (0.3 nM), endotoxin (1 μg/ml) or formyl-Met-Leu-Phe (100 nM) for up to 16 h were not successful. Addition of human PMN primed by GM–CSF (1 nM) to rabbit aortic ring preparations precontracted with phenylephrine had no effect on tone. In contrast to these observations, PMN, isolated from the peritoneum of oyster glycogen treated rats, generatedNO2−via a pathway sensitive to inhibition by the nitric oxide synthase inhibitor, NG-monomethyl L-arginine. However, peripheral blood rat PMN obtained from the same animals did not produceNO2−, even during prolonged incubation for periods of up to 16 h. It is suggested that detectable NO production by PMN requires NO synthase activity to be induced either by the process of PMN migration or by exposure to certain cytokines produced locally at the site of inflammation. |
| Databáze: | OpenAIRE |
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