Computer-aided modeling and optimization for capsaicinoid production by immobilized Capsicum frutescens cells
| Autor: | G. Suvarnalatha, Gokare Aswathanarayana Ravishankar, Nagin Chand, L. V. Venkataraman |
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| Rok vydání: | 1993 |
| Předmět: |
Calcium alginate
Alginates Cytological Techniques chemistry.chemical_element Capsaicinoid Bioengineering Curdlan Calcium Models Biological Applied Microbiology and Biotechnology Biochemistry Matrix (chemical analysis) chemistry.chemical_compound Murashige and Skoog medium Glucuronic Acid Botany Computer Simulation Response surface methodology Plants Medicinal Chromatography Chemistry Hexuronic Acids Glucuronic acid Culture Media Evaluation Studies as Topic Capsaicin Capsicum Biotechnology |
| Zdroj: | Enzyme and Microbial Technology. 15:710-715 |
| ISSN: | 0141-0229 |
| DOI: | 10.1016/0141-0229(93)90074-c |
| Popis: | Production of capsaicinoids by immobilized cell cultures of Capsicum entrapped in calcium alginate matrix is influenced by several factors related to immobilization techniques, including media composition and the biosynthetic capability of cells. Central composite rotatable design (CCRD), one of the response surface methodologies, was used to arrive at optimal conditions for maximizing the yields of capsaicinoids in only 53 experiments, instead of 15,625. Immobilization of cells in 2.5% sodium alginate with 15 min contact time in 60 mM calcium chloride and culture in Murashige and Skoog medium supplemented with 3 mg l-1 2,4-dichlorophenoxyacetic acid and 0.2% curdlan gave the highest yield of 220 micrograms g-1 in 3 days. |
| Databáze: | OpenAIRE |
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