Increased Expression of Interleukin-2 Receptor α on Peripheral Blood Mononuclear Cells in HTLV-I tax/rex mRNA–Positive Asymptomatic Carriers
Autor: | Koichi Murai, Kimitaka Sagawa, Nancy Mueller, Akihiko Okayama, Masayuki Okamoto, Hirohito Tsubouchi, Nobuyoshi Tachibana, Yasuhiro Nagatomo, Takashi Shima, Sherri O. Stuver, Shiro Ishihara |
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Rok vydání: | 1997 |
Předmět: |
Interleukin 2
viruses medicine.medical_treatment Immunology Biology Peripheral blood mononuclear cell Virus Cell Line Virology Gene expression medicine Humans Immunology and Allergy RNA Messenger Messenger RNA Receptors Interleukin-2 Gene Products tax medicine.disease HTLV-I Infections Gene Products rex Leukemia Cytokine Carrier State Leukocytes Mononuclear Asymptomatic carrier medicine.drug |
Zdroj: | Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 15:70-75 |
ISSN: | 1077-9450 |
Popis: | Using the double-nested reverse transcription-polymerase chain reaction, we assayed human T-cell leukemia virus type I (HTLV-I) tax/rex-encoded mRNA in the peripheral blood mononuclear cells (PBMCs) of asymptomatic carriers as an index of the expression of HTLV-I in vivo in relation to the proviral DNA level. HTLV-I tax/rex mRNA was detected in only 1 (3.3%) of 30 samples with medium or lower proviral DNA levels, but it was detected in 11 (39.3%) of 28 samples with high HTLV-I proviral DNA levels, estimated as equal to or more than the proviral DNA of 10 ng of HUT102 (i.e., HUT102 cells were used as positive controls). The mean number of interleukin-2 receptor alpha (IL-2R alpha)-positive cells as a percentage of the total number of PBMCs was higher (13.2%) in the tax/rex mRNA-positive carriers with high proviral DNA levels than in the carriers who were mRNA negative (8.4%) (p = 0.004, Wilcoxon test). These results suggest that virus activation as indicated by the presence of tax/rex mRNA in asymptomatic carriers with high proviral DNA levels is associated with an elevation of the IL-2R alpha-positive cells in vivo. |
Databáze: | OpenAIRE |
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