Aanlysis of dCMP deaminase and CDP reductase levels in hamster cells infected by herpes simplex virus
| Autor: | G Buttin, Y Langelier, M Déchamps |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Ribonucleoside Diphosphate Reductase
viruses Immunology Hamster Virus Replication medicine.disease_cause Thymidine Kinase Microbiology Chinese hamster Cell Line Cricetulus Cricetinae Virology Ribonucleotide Reductases medicine Animals Simplexvirus DCMP Deaminase Cell-Free System biology DCMP deaminase activity biology.organism_classification dCMP deaminase Ribonucleotide reductase Herpes simplex virus Biochemistry Nucleotide Deaminases Thymidine kinase Cell culture Insect Science Mutation Research Article |
| Zdroj: | Journal of Virology. 26:547-553 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.26.3.547-553.1978 |
| Popis: | Several enzymatic activities involved in the biosynthetic pathways of nucleotides, including thymidine kinase, which has been used as a biochemical marker in studies of gene transfer, are induced by herpes simplex virus (HSV). The utility of additional markers prompted us to reanalyze the effects of HSV infection on the activities of two other enzymes for which direct selective methods can be devised: dCMP deaminase and CDP reductase. For this purpose, mutant Chinese hamster (lA1) cells devoid of dCMP deaminase activity or Syrian hamster (BHK-21/C13) cells were infected by HSV type 1 or 2, and the activities of thymidine kinase, dCMP deaminase, and CDP reductase were measured in the cell extracts. The reported induction of thymidine kinase and CDP reductase by HSV was confirmed, whereas the stimulation of dCMP deaminase activity could not be observed. For both cell lines, the HSV-induced CDP reductase differed from the host enzyme by sensitivity to inhibition by both dTTP and dATP. This property should be helpful in developing a selection system for this activity. |
| Databáze: | OpenAIRE |
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