Expression profiles of histone modification genes in gastric cancer progression
| Autor: | Emre Gerçeker, Elmas Kasap, Ufuk Demirci, Fahri Bilgic, Seda Orenay-Boyacioglu, Mehmet Korkmaz, Hakan Yüceyar |
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| Přispěvatelé: | Department of Medical Genetics, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey, Department of Gastroenterology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey, Department of Gastroenterology, Private Gazi Hospital, Izmir, Turkey, Department of Internal Medicine, Faculty of Medicine, Celal Bayar University, Manisa, Turkey, Department of Medical Biology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Gastritis Atrophic Male 0301 basic medicine medicine.disease_cause Histone Deacetylases Helicobacter Infections Histones 03 medical and health sciences 0302 clinical medicine Risk Factors Stomach Neoplasms Genetics medicine Humans NIMA-Related Kinases Epigenetics Molecular Biology Aged Aurora Kinase A Metaplasia Histone deacetylase 5 Helicobacter pylori biology Histone deacetylase 2 Stomach Genetic Variation Cancer General Medicine Middle Aged biology.organism_classification medicine.disease Histone Code Intestines 030104 developmental biology Histone Histone phosphorylation p21-Activated Kinases Gastric Mucosa 030220 oncology & carcinogenesis Disease Progression biology.protein Cancer research Female Transcriptome Carcinogenesis Preliminary Data |
| Zdroj: | Molecular Biology Reports. 45:2275-2282 |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | Gastric cancer (GC) development can be attributed to several risk factors including atrophic gastritis (AG), intestinal metaplasia (IM), and the presence of Helicobacter pylori (HP). Also, histone modification is an epigenetic mechanism that plays a pivotal role in GC carcinogenesis. In this preliminary study, we aimed to describe the expression profiles of histone modification in the AG, IM, and GC patient groups. A total of 80 patients with AG (n = 27), IM (n = 25), and GC (n = 28) with an additional 20 control subjects were included in the study. Expression profiles of three histone phosphorylation genes (PAK1, NEK6, and AURKA) and five histone deacetylation genes (HDACs 1, 2, 3, 5, and 7) were examined based on the results of Real Time qPCR method. It was observed that AURKA and HDAC2 genes were significantly overexpressed in all groups compared to the control (P < 0.05). In GC patients, overexpression of HDAC2 gene was detected in the absence of metastasis, and overexpression of AURKA, HDAC2, and NEK6 genes was detected in the presence of metastasis. When cancer involvements were compared, significant overexpression of the HDAC2 gene was noted in overall and corpus involvements (P < 0.05). In addition, overexpression of AURKA, NEK6, HDAC1, and HDAC2 genes and underexpression of HDAC5 gene were detected in the antrum involvement (P < 0.05). In conclusion, decreased expression of HDAC5 in GC is reported for the first time in this study, while supporting the existing literature in AURKA, NEK6, HDAC1, and HDAC2 up regulations during GC development. © 2018, Springer Nature B.V. |
| Databáze: | OpenAIRE |
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