| Autor: |
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F. |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.19500857 |
| Popis: |
Additional file 2. Recombinant PCR for overexpression of genes Pc-yap1 and Pc-rsmA. The pki gene promoter from A. niger was fused to fragments from the genes extending from the ATG start codon to around 300 bp downstream the TGA stop codon to ensure the presence of the transcriptional terminator. The position of the primers used for PCR reactions is indicated (see Materials and Methods for details). The final fragments with the genes fused to the pki promoter were digested with the restriction enzymes EcoRV and SpeI (Ppki::Pc-yap1) or KpnI and XhoI (Ppki::Pc-rsmA) and inserted in the vector pBKSpyrG to obtain the plasmids pPyrG-pki::Pc-yap1 and pPyrG-pki::Pc-rsmA, respectively; the restriction sites in the primers are highlighted in color. Introns are indicated in grey color. Added restriction sites at the 5’-end of the primers are highlighted in orange color. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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